| Association of Mycobacterium avium subspecies paratuberculosis With Crohn Disease in Pediatric Patients. | |
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MedLine Citation:
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PMID: 21206379 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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OBJECTIVES: : The aim of the present study was to determine the prevalence of Mycobacterium avium subsp paratuberculosis (MAP) DNA in intestinal biopsies from pediatric patients with granulomatous Crohn disease (CD) or ulcerative colitis (UC), and matched control subjects without inflammatory bowel disease (IBD). PATIENTS AND METHODS: : DNA was extracted from formalin-fixed paraffin-embedded colonic and ileal biopsies from patients with CD (n = 22) or UC (n = 20), and from controls without IBD (n = 21). IS900 nested polymerase chain reaction was performed in triplicate to determine the presence of MAP-specific DNA. RESULTS: : In mucosal biopsies from terminal ileum, IS900 amplicons were detected in 1 of 19 (5.2%) control subjects, 1 of 20 (5%) patients with UC, and 7 of 20 (35%) patients with CD (P < 0.05 vs controls, odds ratio 9.6). In colonic biopsies, IS900 amplicons were detected in 0 of 19 control subjects, 1 of 19 (5.2%) patients with UC, and 5 of 19 (26.3%) patients with CD (P < 0.05 vs controls, odds ratio 14.8). In patients with CD, there was no correlation between disease activity and the presence of IS900. CONCLUSIONS: : Our technique enabled sensitive and specific detection of MAP DNA in archival endoscopic biopsy specimens. Although MAP-specific DNA can be detected in about 5% of intestinal biopsies from children with UC or controls without IBD, its presence was significantly associated with pediatric granulomatous CD, being particularly prevalent in ileal tissue. This easily defined clinical subset of patients may be useful for additional studies to determine the role of MAP in CD. |
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Authors:
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Adrienne Lee; Tanya A Griffiths; Rohan S Parab; Robin K King; Marla C Dubinsky; Stefan J Urbanski; Iwona Wrobel; Kevin P Rioux |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of pediatric gastroenterology and nutrition Volume: 52 ISSN: 1536-4801 ISO Abbreviation: J. Pediatr. Gastroenterol. Nutr. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8211545 Medline TA: J Pediatr Gastroenterol Nutr Country: United States |
Other Details:
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Languages: eng Pagination: 170-4 Citation Subset: IM |
Affiliation:
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*Department of Medicine, Division of Gastroenterology, Canada †Department of Pathology and Laboratory Medicine, Canada ‡Department of Pediatrics, Division of Gastroenterology, Faculty of Medicine, University of Calgary, Alberta, Canada §Alberta Agriculture and Rural Development, Edmonton, Alberta, Canada ||UCLA School of Medicine, Pediatric IBD Center, Cedars Sinai Medical Center, Los Angeles, CA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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