Document Detail


Association of the interleukin-2 polymorphisms with interleukin-2 serum levels and risk of nasopharyngeal carcinoma.
MedLine Citation:
PMID:  20438365     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nasopharyngeal carcinoma (NPC) is one of the most common cancers in southern China. In addition to environmental factors such as Epstein-Barr virus infection and chemical carcinogen exposure, genetic susceptibility has been reported to play a key role in the development of this disease. Interleukin-2 (IL-2) is an immunoregulatory cytokine produced by T cells and plays an important role in antitumor immunity. Variations in the DNA sequence of the IL-2 gene may lead to altered cytokine production and/or activity, and thus modulate an individual's susceptibility to NPC. To test this hypothesis, we investigated whether IL-2 gene polymorphisms and its serum levels are associated with NPC in a Chinese population. We analyzed single-nucleotide polymorphisms of IL-2 gene -330 T/G and +114 T/G in 180 patients with NPC and 200 age- and sex-matched controls, using polymerase chain reaction-restriction fragment length polymorphism and DNA sequencing methods, and serum IL-2 levels were measured by enzyme-linked immunosorbent assay. Serum IL-2 levels were decreased in patients with NPC compared with controls (p < 0.01). There were significant differences in the genotype and allele frequencies of IL-2 gene -330 T/G polymorphism between the group of patients with NPC and the control group (p < 0.05). Moreover, genotypes carrying the IL-2 -330 G variant allele were associated with decreased serum IL-2 levels compared with the homozygous wild-type genotype in patients with NPC. Carrying the IL-2 -330 G variant allele was associated with a decreased ability to produce IL-2, which may contribute to NPC susceptibility.
Authors:
Ye-Sheng Wei; Yan Lan; Liang Zhang; Jian-Chu Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  DNA and cell biology     Volume:  29     ISSN:  1557-7430     ISO Abbreviation:  DNA Cell Biol.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-13     Completed Date:  2010-08-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9004522     Medline TA:  DNA Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  363-8     Citation Subset:  IM    
Affiliation:
Department of Medical Laboratory, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China. weiyesheng@yahoo.com.cn
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MeSH Terms
Descriptor/Qualifier:
Alleles
Asian Continental Ancestry Group / genetics
Base Sequence
China / epidemiology
Enzyme-Linked Immunosorbent Assay
Gene Frequency
Genetic Predisposition to Disease
Genotype
Herpesvirus 4, Human / genetics
Homozygote
Humans
Interleukin-2 / genetics*
Nasopharyngeal Neoplasms / epidemiology,  genetics*,  pathology
Polymerase Chain Reaction
Polymorphism, Genetic*
Polymorphism, Restriction Fragment Length
Polymorphism, Single Nucleotide
Risk Factors
Chemical
Reg. No./Substance:
0/Interleukin-2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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