Document Detail

Association of Escherichia coli O157:H7 with houseflies on a cattle farm.
MedLine Citation:
PMID:  15574966     Owner:  NLM     Status:  MEDLINE    
The ecology of Escherichia coli O157:H7 is not well understood. The aims of this study were to determine the prevalence of and characterize E. coli O157:H7 associated with houseflies (HF). Musca domestica L. HF (n = 3,440) were collected from two sites on a cattle farm over a 4-month period and processed individually for E. coli O157:H7 isolation and quantification. The prevalence of E. coli O157:H7 was 2.9 and 1.4% in HF collected from feed bunks and a cattle feed storage shed, respectively. E. coli O157:H7 counts ranged from 3.0 x 10(1) to 1.5 x 10(5) CFU among the positive HF. PCR analysis of the E. coli O157:H7 isolates revealed that 90.4, 99.2, 99.2, and 100% of them (n = 125) possessed the stx1, stx2, eaeA, and fliC genes, respectively. Large populations of HF on cattle farms may play a role in the dissemination of E. coli O157:H7 among animals and to the surrounding environment.
Muhammad J Alam; Ludek Zurek
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  70     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-12-02     Completed Date:  2005-02-15     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7578-80     Citation Subset:  IM    
Department of Entomology, Kansas State University, Manhattan, KS 66506, USA.
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MeSH Terms
Animal Feed
Escherichia coli O157 / classification,  genetics,  isolation & purification*
Houseflies / microbiology*
Polymerase Chain Reaction

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