| Association of Escherichia coli O157:H7 with houseflies on a cattle farm. | |
| | |
MedLine Citation:
|
PMID: 15574966 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The ecology of Escherichia coli O157:H7 is not well understood. The aims of this study were to determine the prevalence of and characterize E. coli O157:H7 associated with houseflies (HF). Musca domestica L. HF (n = 3,440) were collected from two sites on a cattle farm over a 4-month period and processed individually for E. coli O157:H7 isolation and quantification. The prevalence of E. coli O157:H7 was 2.9 and 1.4% in HF collected from feed bunks and a cattle feed storage shed, respectively. E. coli O157:H7 counts ranged from 3.0 x 10(1) to 1.5 x 10(5) CFU among the positive HF. PCR analysis of the E. coli O157:H7 isolates revealed that 90.4, 99.2, 99.2, and 100% of them (n = 125) possessed the stx1, stx2, eaeA, and fliC genes, respectively. Large populations of HF on cattle farms may play a role in the dissemination of E. coli O157:H7 among animals and to the surrounding environment. |
| | |
Authors:
|
Muhammad J Alam; Ludek Zurek |
Related Documents
:
|
15288926 - Characterisation of attaching-effacing escherichia coli isolated from animals at slaugh... 9546176 - Longitudinal study of escherichia coli o157:h7 dissemination on four dairy farms in wis... 11123466 - Modelling inactivation of escherichia coli by low ph: application to passage through th... 9172336 - Epidemiological relatedness and clonal types of natural populations of escherichia coli... 10386456 - Minicircle variable region probes for characterization of leishmania (viannia) species. 7790086 - Heterologous antigen expression in vibrio cholerae vector strains. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
|
Title: Applied and environmental microbiology Volume: 70 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2004 Dec |
Date Detail:
|
Created Date: 2004-12-02 Completed Date: 2005-02-15 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
|
Languages: eng Pagination: 7578-80 Citation Subset: IM |
Affiliation:
|
Department of Entomology, Kansas State University, Manhattan, KS 66506, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Agriculture* Animal Feed Animals Cattle Escherichia coli O157 / classification, genetics, isolation & purification* Houseflies / microbiology* Polymerase Chain Reaction Prevalence Seasons |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Genotypes of Cryptosporidium species infecting fur-bearing mammals differ from those of species infe...
Next Document: Implementation of standards for individual monitoring in Europe.