Document Detail

Association of CC chemokine receptor 7 with lymph node metastasis of esophageal squamous cell carcinoma.
MedLine Citation:
PMID:  12960129     Owner:  NLM     Status:  MEDLINE    
PURPOSE: CC chemokine receptor 7 (CCR7) plays a critical role in the migration of activated dendritic cells to regional lymph nodes. Recent studies have shown that CCR7 is involved in metastasis in some malignant diseases. The role of CCR7 in esophageal squamous cell carcinoma (SCC) has not yet been clarified. EXPERIMENTAL DESIGN: We performed reverse transcription-PCR analysis for CCR7 in 20 esophageal SCC cell lines and immunohistochemical analysis of 96 esophageal SCC samples. We then performed a cell migration assay, F-actin polymerization, and a phagokinetic assay on esophageal SCC cell lines in the presence of CCL21, a ligand of CCR7. RESULTS: CCR7 mRNA was detected in 9 of 20 esophageal SCC cell lines. Immunoreactive CCR7 was found mainly in esophageal cancer cells. High CCR7 expression was significantly correlated with esophageal SCC lymphatic permeation, lymph node metastasis, tumor depth, and tumor-node-metastasis stage and was associated with poor survival. In vitro studies demonstrated that CCL21 significantly increased the cell migration ability of esophageal SCC cell lines, and pseudopodia formation was induced by CCL21 stimulation. Furthermore, CCL21 markedly enhanced the motility of esophageal carcinoma cell lines by the phagokinetic assay. CONCLUSIONS: The results suggested that the CCR7/CCL21 receptor ligand system may play a role in the lymph node metastasis of esophageal SCC.
Yongzeng Ding; Yutaka Shimada; Masato Maeda; Atsushi Kawabe; Junichi Kaganoi; Izumi Komoto; Yosuke Hashimoto; Masayuki Miyake; Hiroki Hashida; Masayuki Imamura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical cancer research : an official journal of the American Association for Cancer Research     Volume:  9     ISSN:  1078-0432     ISO Abbreviation:  Clin. Cancer Res.     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-09-08     Completed Date:  2004-05-14     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9502500     Medline TA:  Clin Cancer Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3406-12     Citation Subset:  IM    
Department of Surgery and Surgical Basic Science, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan.
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MeSH Terms
Carcinoma, Squamous Cell / pathology*
Cell Line, Tumor
Cell Movement
Dendritic Cells / metabolism
Esophageal Neoplasms / pathology*
Lymphatic Metastasis*
Microscopy, Confocal
Microscopy, Fluorescence
Middle Aged
RNA / metabolism
RNA, Messenger / metabolism
Receptors, CCR7
Receptors, Chemokine / biosynthesis*
Reverse Transcriptase Polymerase Chain Reaction
Time Factors
Reg. No./Substance:
0/CCR7 protein, human; 0/Ligands; 0/RNA, Messenger; 0/Receptors, CCR7; 0/Receptors, Chemokine; 63231-63-0/RNA

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