Document Detail

Association between an emerging disseminated form of leishmaniasis and Leishmania (Viannia) braziliensis strain polymorphisms.
MedLine Citation:
PMID:  23035200     Owner:  NLM     Status:  MEDLINE    
Leishmania (Viannia) braziliensis causes three main types of American tegumentary leishmaniasis (ATL), localized cutaneous leishmaniasis (CL), mucosal leishmaniasis (ML), and disseminated leishmaniasis (DL). All forms are observed among individuals of Corte de Pedra, Brazil. We previously used random amplified markers to identify a multiclonal population among L. (V.) braziliensis isolates from ATL patients, defining parasite clades associated with different clinical syndromes. Herein we compared sequences of random amplified markers to identify genotypes of L. (V.) braziliensis recovered from lesions of CL, ML, and DL patients. Six polymorphic genomic loci were sequenced from 35 parasite isolates. Single-nucleotide polymorphisms (SNPs) and insertions-deletions (indels) at each locus allowed us to segregate the L. (V.) braziliensis population according to haplotypes. Several SNPs, indels, and haplotypes were significantly associated with an increased risk of DL. Molecular genotyping may provide markers to identify L. (V.) braziliensis strains likely to cause this emerging, hard-to-treat form of ATL.
Adriano Queiroz; Rosana Sousa; Claudia Heine; Manuela Cardoso; Luiz Henrique Guimarães; Paulo Roberto Lima Machado; Edgar M Carvalho; Lee W Riley; Mary E Wilson; Albert Schriefer
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-10-03
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  50     ISSN:  1098-660X     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-19     Completed Date:  2013-04-17     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4028-34     Citation Subset:  IM    
Serviço de Imunologia, Hospital Universitário Professor Edgard Santos, Universidade Federal da Bahia, Salvador, Bahia, Brazil.
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MeSH Terms
DNA, Protozoan / genetics
Genetic Variation*
Leishmania braziliensis / classification*,  genetics,  isolation & purification*
Leishmaniasis / parasitology*,  pathology*
Random Amplified Polymorphic DNA Technique
Sequence Analysis, DNA
Grant Support
P50-AI30639-16/AI/NIAID NIH HHS; R03-A167663-01//PHS HHS; R24 TW007988/TW/FIC NIH HHS
Reg. No./Substance:
0/DNA, Protozoan

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