Document Detail


Association of BCL-2 with membrane hyperpolarization and radioresistance.
MedLine Citation:
PMID:  8647905     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The resting membrane potential of parental, neomycin control, and Bcl-2 transfected cells was measured, and the effect of membrane hyperpolarization or depolarization on radiosensitivity was studied. Bcl-2 transfected cells were significantly more radioresistant than control cells and were significantly hyperpolarized compared to parental and neomycin control transfected PW and HL60 cells. Hyperpolarization of the parental and neomycin control transfected cells by valinomycin significantly increased the radioresistance of these cells to such an extent that there was no longer a significant difference in the survival of the valinomycin treated and irradiated control cells compared to similarly irradiated Bcl-2 transfected cells. In contrast, depolarization of the Bcl-2 transfected PW and HL60 cells decreased the radioresistance of the Bcl-2 transfectants to a level similar to that of the control cells. The data presented here suggest that overexpression of Bcl-2 affects membrane potential and that this hyperpolarization is associated with increased radioresistance of cells that overexpress Bcl-2. Furthermore, Bcl-2 transfected cells were also less susceptible to the specific Na+/K(+)-ATPase inhibitor ouabain, suggesting that Bcl-2 may act at the level of the Na+/K(+)-ATPase pump.
Authors:
M S Gilbert; A H Saad; B A Rupnow; S J Knox
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  168     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  1996 Jul 
Date Detail:
Created Date:  1996-07-25     Completed Date:  1996-07-25     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  114-22     Citation Subset:  IM    
Affiliation:
Department of Radiation Oncology, Stanford University Medical Center, California 94305-5105, USA.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects
Cell Membrane / physiology*
Cell Survival / drug effects,  radiation effects
HL-60 Cells / physiology
Humans
Lymphoma, B-Cell
Membrane Potentials
Ouabain / pharmacology
Potassium Chloride / pharmacology
Proto-Oncogene Proteins / physiology*
Proto-Oncogene Proteins c-bcl-2
Sodium-Potassium-Exchanging ATPase / antagonists & inhibitors
Tumor Cells, Cultured / radiation effects*
Valinomycin / pharmacology
Grant Support
ID/Acronym/Agency:
CA09302/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 2001-95-8/Valinomycin; 630-60-4/Ouabain; 7447-40-7/Potassium Chloride; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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