Document Detail


Assisted Hatching of Poor-quality Bovine Embryos Increases Pregnancy Success Rate after Embryo Transfer.
MedLine Citation:
PMID:  21606632     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Embryos of good, fair and poor quality were collected from superovulated cows and subjected to zona cutting (ZC) treatment using a needle under either an inverted microscope or a stereomicroscope. One (single transfer) or 2 (twin transfer) embryos with or without prior ZC treatment were transferred nonsurgically to recipients. Without the ZC treatment, lower embryonic quality resulted in lower pregnancy success rates. However, the ZC treatment increased the pregnancy success rate following transfer of poor-quality embryos, but not the pregnancy rate after transfer of good- or fair-quality embryos. No differences were observed between the pregnancy success rates after the transfer of embryos treated under the inverted microscope and those after transfer of embryos treated under the stereomicroscope, and this was the same after single and twin transfer. Moreover, ZC treatment of embryos prior to transfer did not result in an increased abortion rate, irrespective of the number of transferred embryos. In conclusion, ZC treatment improves pregnancy success rates following transfer of poor-quality embryos. Moreover, the results indicate that ZC treatment by using a stereomicroscope is practical for on-farm application.
Authors:
Atsushi Taniyama; Yasutaka Watanabe; Youji Nishino; Tetsurou Inoue; Yasuho Taura; Mitsuhiro Takagi; Chikara Kubota; Takeshige Otoi
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-5-23
Journal Detail:
Title:  The Journal of reproduction and development     Volume:  -     ISSN:  1348-4400     ISO Abbreviation:  -     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-5-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9438792     Medline TA:  J Reprod Dev     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Nagasaki Prefectural Livestock Experiment Station.
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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