| Assimilation of benzene carbon through multiple trophic levels traced by different stable isotope probing methodologies. | |
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MedLine Citation:
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PMID: 21517916 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The flow of benzene carbon along a food chain consisting of bacteria and eukaryotes, including larvae (Diptera: Chironomidae) was evaluated by total lipid fatty acids (TLFAs)-, amino acid- and protein-stable isotope probing (SIP). A coconut-fiber textile, colonized by a benzene-degrading biofilm, was sampled in a system established for the remediation of benzene, toluene, ethylbenzene and xylenes (BTEX) polluted groundwater, and incubated with (12) C- and [(13) C(6) ]-benzene (>99atom%) in a batch-scale experiment for 2 to 8 days. After 8 days, Chironomus sp. larvae were added to study carbon flow to higher trophic levels. Gas chromatography-combustion-isotope ratio monitoring mass spectrometry of TLFA showed increased isotope ratios in the (13) C-benzene incubated-biofilm. A higher (13) C-enrichment was observed in TLFAs indicative of Gram-negative bacteria than for Gram-positive. Fatty acid indicators of eukaryotes showed significant (13) C-incorporation but to lower extent than bacterial indicators. Fatty acids extracted from larvae feeding on (13) C-biofilm reached isotopic ratio of 1.55 atom% illustrating that the larvae feed to some extent on labelled biomass. No (13) C-incorporation was detectable in larval proteins after their separation by sodium-dodecyl sulphate-polyacrylamide gel electrophoresis and analysis by nano-liquid-chromatography-mass spectrometry. The flow of benzene derived carbon could be traced in a food web consisting of bacteria and eukaryotes. |
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Authors:
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F Bastida; S Jechalke; P Bombach; A G Franchini; J Seifert; M von Bergen; C Vogt; H H Richnow |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-4-21 |
Journal Detail:
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Title: FEMS microbiology ecology Volume: - ISSN: 1574-6941 ISO Abbreviation: - Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-4-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8901229 Medline TA: FEMS Microbiol Ecol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved. |
Affiliation:
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Department of Isotope Biogeochemistry, UFZ - Helmholtz Centre for Environmental Research, Leipzig, Germany Department of Environmental Biotechnology, UFZ - Helmholtz Centre for Environmental Research, Leipzig, Germany Department of Proteomics, UFZ - Helmholtz Centre for Environmental Research, Leipzig, Germany Department of Metabolomics, UFZ - Helmholtz Centre for Environmental Research, Leipzig, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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