Document Detail

Assignment of the human moesin gene (MSN) to chromosome region Xq11.2-->q12.
MedLine Citation:
PMID:  8125014     Owner:  NLM     Status:  MEDLINE    
The human moesin gene (MSN) was mapped to the long arm of the X chromosome. PCR products for the moesin gene cDNA were used as probes to isolate their corresponding cosmid clones. Fluorescence in situ hybridization (FISH) with two of the isolated cosmid probes showed signals at Xq11.2-->q12, whereas four other cosmids showed FISH signals on chromosome 5. Southern blot hybridization, using a PCR product corresponding to the 3' region of the moesin gene cDNA as a probe (probe-3), on one of the two cosmids that produced signals on the X chromosome gave 5.7- and 3.5-kb HindIII fragments. Further Southern hybridization of the DNA from XY, XX, and XXXXX individuals using probe-3 revealed a gene-dose effect of the X chromosome on the size of a 3.5-kb and a 3.0-kb HindIII fragment; in contrast, an invariant 9.8-kb band was present in the DNA of all individuals tested. Sequencing of an exon-intron border revealed that the two cosmids had predicted sequences. These results indicated that the two cosmids contained MSN, and it was consequently assigned to human chromosome region Xq11.2-->q12. These results strongly suggest that MSN may be removed from candidacy for Wiskott-Aldrich syndrome, which has been putatively mapped to Xp11.3-->p11.22.
T Kishino; T Ariga; H Soejima; T Tamura; T Ohta; Y Jinno; S Yonemura; N Sato; S Tsukita; S Tsukita
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytogenetics and cell genetics     Volume:  66     ISSN:  0301-0171     ISO Abbreviation:  Cytogenet. Cell Genet.     Publication Date:  1994  
Date Detail:
Created Date:  1994-04-14     Completed Date:  1994-04-14     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0367735     Medline TA:  Cytogenet Cell Genet     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  167-9     Citation Subset:  IM    
Department of Human Genetics, Nagasaki University School of Medicine, Japan.
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MeSH Terms
Chromosome Mapping
DNA, Complementary
In Situ Hybridization, Fluorescence
Linkage (Genetics)
Microfilament Proteins*
Proteins / genetics*
Wiskott-Aldrich Syndrome / genetics
X Chromosome*
Reg. No./Substance:
0/DNA, Complementary; 0/Microfilament Proteins; 0/Proteins; 144131-77-1/moesin

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