| Assignment of fluorine nuclear magnetic resonance signals from rabbit cyanomethemoglobin. | |
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MedLine Citation:
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PMID: 6305406 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A fluorine NMR study of cyanomethemoglobin prepared from hemoglobin isolated from rabbits maintained on a diet containing DL-p-fluorophenylalanine is described. The results indicate that substitution of fluorophenylalanine occurs essentially randomly at all phenylalanine positions of the alpha- and beta-globin chains; a set of hybrid hemoglobins in which only the alpha- or only the beta-chains contain the fluorinated amino acid was prepared and used to ascertain the fluorine NMR signals arising from each chain. The temperature and pH dependences of chemical shifts, spin-lattice relaxation times, 19F(1H) nuclear Overhauser effects, and the effect of chemical modification of the beta-93 sulfhydryl groups were examined. When considered in light of presently available X-ray structures of human and horse hemoglobins, the available data permit a tentative assignment of most signals to particular fluorophenylalanine/phenylalanine positions in the globin sequences. |
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Authors:
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J T Gerig; J C Klinkenborg; R A Nieman |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemistry Volume: 22 ISSN: 0006-2960 ISO Abbreviation: Biochemistry Publication Date: 1983 Apr |
Date Detail:
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Created Date: 1983-08-11 Completed Date: 1983-08-11 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2076-87 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis Animals Electron Spin Resonance Spectroscopy Hemoglobins Hydrogen-Ion Concentration Magnetic Resonance Spectroscopy / methods Mathematics Methemoglobin / analogs & derivatives*, analysis Peptide Fragments / analysis Protein Multimerization Rabbits Tyrosine p-Fluorophenylalanine / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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GM-25975/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Hemoglobins; 0/Peptide Fragments; 0/cyanomethemoglobin; 55520-40-6/Tyrosine; 60-17-3/p-Fluorophenylalanine; 9008-37-1/Methemoglobin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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