| Assignment of NH resonances in nucleic acids using natural abundance 15N-1H correlation spectroscopy with spin-echo and gradient pulses. | |
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MedLine Citation:
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PMID: 7688695 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It is well known that 2D 15N-1H correlation spectra can resolve overlapped imino proton resonances in the downfield NMR spectra of nucleic acids according to their 15N chemical shifts, and that these resonances can be assigned by base type on that basis, independent of conformation. This information can be extremely important in determining the solution structure of a nucleic acid by NMR, but previously could only be obtained using 15N-labeled, or very concentrated samples. Here we report the design of a gradient-enhanced, jump-return spin echo version of an 15N-1H HMQC experiment (GE-JRSE HMQC) that is sensitive enough to work on unlabeled nucleic acid samples at normal NMR concentrations. This experiment has led to the assignment of imino proton resonances with non-Watson Crick chemical shifts in the spectrum of a 29 residue oligoribonucleotide that models the sarcin/ricin loop from 28S rRNA. |
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Authors:
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A A Szewczak; G W Kellogg; P B Moore |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: FEBS letters Volume: 327 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1993 Aug |
Date Detail:
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Created Date: 1993-09-13 Completed Date: 1993-09-13 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 261-4 Citation Subset: IM |
Affiliation:
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Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Imino Acids / chemistry Magnetic Resonance Spectroscopy / methods* Molecular Sequence Data Nitrogen Isotopes Nucleic Acid Conformation Protons RNA / chemistry* Rats |
| Grant Support | |
ID/Acronym/Agency:
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GM-41651/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Imino Acids; 0/Nitrogen Isotopes; 0/Protons; 63231-63-0/RNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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