| Assessment of visceral pain-related pseudo-affective responses to colorectal distension in mice by intracolonic manometric recordings. | |
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MedLine Citation:
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PMID: 16459276 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recently, a new manometric method has been proposed to quantify visceromotor responses (VMR) to colorectal distension (CRD) in rats. This method is based on monitoring pressure changes within the distending balloon during CRD. This study assesses the applicability of such a technique to the quantification of VMRs to CRD in mice. Electrical activity of the abdominal muscles and pressure changes within the distending balloon (mechanical response) were simultaneously recorded in conscious mice during CRD (phasic ascending, 10-80 mm Hg, or repetitive, 55 mm Hg). There was a clear stimulus-response relationship with a strong correlation between electrical and mechanical responses during the ascending (r(2) = 0.899, n = 7) or repetitive phasic CRD (r(2) = 0.926, n = 8). Repetitive phasic distensions (55 mm Hg) increased the mechanical and electrical responses by 71 +/- 20% and 42 +/- 16%, respectively (pulses 10-12 vs. 1-3; n = 8, both P < .01). Atropine (0.5 or 1 mg/kg, subcutaneously) did not affect the mechanical response to CRD. The mu-opioid agonist, fentanyl (0.05 mg/kg, subcutaneously), completely prevented the sensitizing response associated to repetitive distensions. These results show that noninvasive, surgery-free manometry of intracolonic pressure is a reliable method to assess VMRs to CRD in mice. The analgesic effect of compounds could be determined, indicating that the method can be used in pharmacologic studies. PERSPECTIVE: The model presented to assess visceral pain in mice allows a broad use of this species in pharmacological studies and will be of use in the characterization of potential targets and new drugs for the treatment of human pathologies with visceral pain arising from the gut as a significant component. |
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Authors:
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Susanne Arvidsson; Marie Larsson; Håkan Larsson; Erik Lindström; Vincente Martinez |
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Publication Detail:
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Type: Journal Article; Validation Studies |
Journal Detail:
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Title: The journal of pain : official journal of the American Pain Society Volume: 7 ISSN: 1526-5900 ISO Abbreviation: J Pain Publication Date: 2006 Feb |
Date Detail:
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Created Date: 2006-02-06 Completed Date: 2006-05-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100898657 Medline TA: J Pain Country: United States |
Other Details:
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Languages: eng Pagination: 108-18 Citation Subset: IM |
Affiliation:
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Integrative Pharmacology-Gastrointestinal Biology, AstraZeneca R&D Mölndal, Mölndal, Sweden. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Abdominal Muscles
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physiopathology* Abdominal Pain / etiology, physiopathology*, psychology Animals Colonic Diseases / complications, physiopathology* Dilatation, Pathologic / complications, physiopathology Disease Models, Animal Electromyography Female Manometry / methods* Mice Mice, Inbred C57BL Motor Activity / physiology* Muscle Contraction / physiology* Reproducibility of Results |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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