| Assessment system for dioxin absorption in the small intestine and prevention of its absorption by food factors. | |
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MedLine Citation:
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PMID: 15630229 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It has been reported that 90% of the amount of dioxin in the whole body is absorbed orally with food. However, a concise and simple system to assess dioxin absorption in the small intestine has not yet been established. The present study reports a new in vitro assessment system for this purpose. A stable dioxin-responsive cell line was established by introducing a plasmid that incorporates a xenobiotic-responsive element upstream of the luciferase gene into human hepatic HepG2 genomic DNA. Dioxin was added to the apical side of differentiated human intestinal epithelial Caco-2 cell monolayers that had been cultured on a semipermeable membrane, and the basal medium was recovered after an appropriate incubation time. To the recovered medium was added dioxin-responsive HepG2, and a luciferase assay was performed. The established stable cell line clearly showed dose-and time-dependent response to dioxin. When a food factor such as chlorophyll, which has been reported to increase dioxin excretion in in vivo studies, was added with dioxin, a significant decrease in dioxin permeability to the Caco-2 monolayer was observed. This assessment system would be useful to search for those food factors that could prevent dioxin absorption in the small intestine. |
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Authors:
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Yayoi Natsume; Hideo Satsu; Kazushige Kitamura; Naoto Okamoto; Makoto Shimizu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: BioFactors (Oxford, England) Volume: 21 ISSN: 0951-6433 ISO Abbreviation: Biofactors Publication Date: 2004 |
Date Detail:
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Created Date: 2005-01-04 Completed Date: 2005-04-18 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8807441 Medline TA: Biofactors Country: Netherlands |
Other Details:
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Languages: eng Pagination: 375-7 Citation Subset: IM |
Affiliation:
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Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Carcinoma, Hepatocellular Cell Differentiation Cell Line, Tumor Colonic Neoplasms Cytochrome P-450 CYP1A1 / genetics Dioxins / pharmacokinetics* Humans Intestinal Absorption / physiology* Intestinal Mucosa Intestine, Small / physiology* Liver Neoplasms Plasmids Promoter Regions, Genetic Transfection |
| Chemical | |
Reg. No./Substance:
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0/Dioxins; EC 1.14.14.1/Cytochrome P-450 CYP1A1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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