Document Detail


Assessment system for dioxin absorption in the small intestine and prevention of its absorption by food factors.
MedLine Citation:
PMID:  15630229     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It has been reported that 90% of the amount of dioxin in the whole body is absorbed orally with food. However, a concise and simple system to assess dioxin absorption in the small intestine has not yet been established. The present study reports a new in vitro assessment system for this purpose. A stable dioxin-responsive cell line was established by introducing a plasmid that incorporates a xenobiotic-responsive element upstream of the luciferase gene into human hepatic HepG2 genomic DNA. Dioxin was added to the apical side of differentiated human intestinal epithelial Caco-2 cell monolayers that had been cultured on a semipermeable membrane, and the basal medium was recovered after an appropriate incubation time. To the recovered medium was added dioxin-responsive HepG2, and a luciferase assay was performed. The established stable cell line clearly showed dose-and time-dependent response to dioxin. When a food factor such as chlorophyll, which has been reported to increase dioxin excretion in in vivo studies, was added with dioxin, a significant decrease in dioxin permeability to the Caco-2 monolayer was observed. This assessment system would be useful to search for those food factors that could prevent dioxin absorption in the small intestine.
Authors:
Yayoi Natsume; Hideo Satsu; Kazushige Kitamura; Naoto Okamoto; Makoto Shimizu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  BioFactors (Oxford, England)     Volume:  21     ISSN:  0951-6433     ISO Abbreviation:  Biofactors     Publication Date:  2004  
Date Detail:
Created Date:  2005-01-04     Completed Date:  2005-04-18     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8807441     Medline TA:  Biofactors     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  375-7     Citation Subset:  IM    
Affiliation:
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
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MeSH Terms
Descriptor/Qualifier:
Carcinoma, Hepatocellular
Cell Differentiation
Cell Line, Tumor
Colonic Neoplasms
Cytochrome P-450 CYP1A1 / genetics
Dioxins / pharmacokinetics*
Humans
Intestinal Absorption / physiology*
Intestinal Mucosa
Intestine, Small / physiology*
Liver Neoplasms
Plasmids
Promoter Regions, Genetic
Transfection
Chemical
Reg. No./Substance:
0/Dioxins; EC 1.14.14.1/Cytochrome P-450 CYP1A1

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