Document Detail


Assessment of spermatogenesis through staging of seminiferous tubules.
MedLine Citation:
PMID:  22992924     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Male germ cells in all mammals are arranged within the seminiferous epithelium of the testicular tubules in a set of well-defined cell associations called stages. The cellular associations found in these stages and characteristics of the cells used to identify the stages have been well described. Here we present a binary decision key roadmap for identifying stages and present several examples of how staging tubules can be used to better assess the developmental profile of gene expression during spermatogenesis and defects in spermatogenesis arising in pathological conditions resulting from genetic mutations in mice. In particular, when one or more cells of a cellular association cannot be clearly identified or are missing, the cell types that should be present can be precisely identified by knowledge of the approximate or exact stage of the tubule cross section.
Authors:
Marvin L Meistrich; Rex A Hess
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  927     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-09-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  299-307     Citation Subset:  IM    
Affiliation:
Department of Experimental Radiation Oncology, University of Texas M. D. Anderson Cancer Center, Houston, TX, USA, meistrich@mdanderson.org.
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