Document Detail


Assessment of the sequential simulated gastrointestinal tolerance of lactic acid bacteria from kefir grains by response surface methodology.
MedLine Citation:
PMID:  19723219     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The tolerance of lactic acid bacteria (LAB) from kefir grains to gastrointestinal tract conditions was evaluated in vitro. The effects of pH values and bile salts on the viability of LAB were investigated. The results demonstrated that pH value showed a significant effect on the viability. The viable counts exhibited a reduction of 1.5 to 2 log cycles in 0.3% to 0.5% bile salts after 4 h. The viability of LAB exposure to sequential simulated gastric and intestinal juices was assessed by response surface model (RSM). RSM indicated that the gastric pH and gastric contact time significantly affected the viability (P < 0.05), while the effect of intestinal contact time was not significant. Moreover, RSM revealed the interactions of pH and gastric contact time, and of pH and intestinal contact time. The LAB cells, temporarily damaged by the low pH of gastric juice (pH < 2), could recover in the intestinal juice; and the longer the intestinal contact time, the higher the viability of LAB. RSM proved to be a useful and accurate method to predict the viability of LAB under certain laboratory conditions by the model validation. This study indicated that LAB from kefir grains exhibited excellent tolerance to sequential simulated gastrointestinal tract conditions, and that kefiran possessed a significant protective effect on LAB in hostile environments.
Authors:
Tong Zhou; Bo Li; Cheng Peng; Bao-Ping Ji; Gang Chen; Ya-Li Ren
Related Documents :
15035379 - Viability of acid-adapted escherichia coli o157:h7 in ground beef treated with acidic c...
16347119 - Intracellular conditions required for initiation of solvent production by clostridium a...
2551179 - Ph regulation in hepatoma cells: roles for na-h exchange, cl-hco3 exchange, and na-hco3...
3225749 - Isoelectric points of some sulfonamides: determination by microelectrophoresis and by c...
10557239 - The correlation of profiles of surface ph and elongation growth in maize roots.
20174699 - Spatial changes in acid secretion from isolated stomach tissue using a ph-histamine sen...
7806149 - The role of sodium in the uptake of ursodeoxycholic acid in isolated hamster hepatocytes.
15704899 - Versatile solid-phase synthesis of peptide-derived 2-oxazolines. application in the syn...
16346459 - Microbial transformation of esters of chlorinated carboxylic acids.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of food science     Volume:  74     ISSN:  1750-3841     ISO Abbreviation:  J. Food Sci.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-09-02     Completed Date:  2010-01-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0014052     Medline TA:  J Food Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  M328-34     Citation Subset:  IM    
Affiliation:
College of Food Science and Nutritional Engineering, China Agricultural Univ., Haidian District, Beijing 100083, People's Republic of China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Bacteria / growth & development*
Bacterial Physiological Phenomena
Bile Acids and Salts / physiology
Cattle
Colony Count, Microbial
Computer Simulation
Cultured Milk Products / microbiology*,  physiology
Digestion
Freeze Drying
Gastric Juice / chemistry,  enzymology
Gastrointestinal Contents / chemistry,  enzymology
Gastrointestinal Tract / microbiology*
Hydrogen-Ion Concentration
Intestinal Secretions / chemistry,  enzymology
Lactobacillales / growth & development*
Lactococcus / growth & development*
Microbial Viability
Milk
Polysaccharides / isolation & purification,  physiology*
Probiotics
Time Factors
Chemical
Reg. No./Substance:
0/Bile Acids and Salts; 0/Polysaccharides; 0/kefir grain polysaccharide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effectiveness of cross-flow microfiltration for removal of microorganisms associated with unpasteuri...
Next Document:  Biopreservation of refrigerated and vacuum-packed Dicentrarchus labrax by lactic acid bacteria.