Document Detail


Assessment of respiratory hypersensitivity in guinea pigs sensitized to toluene diisocyanate: improvements on analysis of respiratory response.
MedLine Citation:
PMID:  9441717     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Groups of guinea pigs of the Hartley strain were sensitized to toluene diisocyanate (TDI) by combined single intradermal injection and repeated inhalation exposure (3 h/day for 5 consecutive days) to 0, 3.8, 11, 26, 46, and 51 mg TDI/m3 air. One group of animals was sensitized by intradermal injection only. Sham-exposed and TDI-polyisocyanate resin-sensitized guinea pigs served as controls. Three weeks after the first encounter with the inducing agent, animals were challenged with the free TDI (approximately 0.5 mg/m3) and 1 week later with TDI-guinea pig serum albumin conjugate. Breathing patterns were analyzed by objective mathematical procedures taking into account the intensity and duration of the respiratory rate exceeding +/- 3 standard deviations of the individual prechallenge exposure period. In none of the animals challenged with TDI were conclusive immediate-onset respiratory responses identified. During the TDI conjugate challenge a characteristic increase in respiratory rate was observed in all groups sensitized with TDI. In each of the sham and TDI-resin control groups, 1 of 16 animals responded mildly to the conjugate challenge. With regard to analysis of the development of asthma-like dyspnea, the results obtained suggest that respiratory response can suitably be defined by objective mathematical analysis of breathing patterns. Moreover, the "duration" of response exceeding +3 x standard deviation of prechallenge baseline data appears to show less variability when compared to the "intensity" of response (area). It can be concluded that this method of evaluation of respiratory response may be useful to compare more quantitatively this type of data and serves the objective of decreasing potential interlaboratory variability.
Authors:
J Pauluhn
Related Documents :
24195617 - Serial assessment of local peripheral vascular function after eccentric exercise.
21679117 - Proteomic responses of skeletal and cardiac muscle to exercise.
12212977 - Exhaled nitric oxide and bronchial responsiveness in healthy subjects exposed to organi...
1489527 - Acute toxic effects of mercuric chloride on the mucocytes of the epithelial lining of t...
17890107 - Biceps brachii myoelectric and oxygenation changes during static and sinusoidal isometr...
2745817 - Nutritive value of high moisture alfalfa hay preserved with urea.
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Fundamental and applied toxicology : official journal of the Society of Toxicology     Volume:  40     ISSN:  0272-0590     ISO Abbreviation:  Fundam Appl Toxicol     Publication Date:  1997 Dec 
Date Detail:
Created Date:  1998-03-09     Completed Date:  1998-03-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8200838     Medline TA:  Fundam Appl Toxicol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  211-9     Citation Subset:  IM    
Affiliation:
Institute of Toxicology, BAYER AG, Wuppertal, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Administration, Inhalation
Allergens / administration & dosage,  toxicity*
Animals
Disease Models, Animal
Dose-Response Relationship, Drug
Female
Guinea Pigs
Hypersensitivity, Delayed / chemically induced,  immunology
Injections, Intradermal
Mathematics
Reproducibility of Results
Respiration / drug effects,  immunology*
Respiratory Hypersensitivity / chemically induced*,  immunology*
Time Factors
Toluene 2,4-Diisocyanate / administration & dosage,  immunology,  toxicity*
Chemical
Reg. No./Substance:
0/Allergens; 584-84-9/Toluene 2,4-Diisocyanate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Rat olfactory mucosa displays a high activity in metabolizing methyl tert-butyl ether and other gaso...
Next Document:  Intratracheal inhalation vs intratracheal instillation: differences in particle effects.