Document Detail


Assessment of removal of human cytomegalovirus from blood components by leukocyte depletion filters using real-time quantitative PCR.
MedLine Citation:
PMID:  14525779     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To assess removal of cytomegalovirus (CMV) by leukocyte depletion (LD) filters, we developed a spiking model of latent virus using peripheral blood mononuclear cells (PBMCs) infected by coculture with CMV-infected human fibroblasts. Infected PBMCs were purified by dual magnetic column selection and then spiked into whole blood units or buffy coat pools prior to LD by filtration. CMV load and fibroblast contamination were assessed using quantitative CMV DNA real-time PCR and quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) of mRNA encoding the fibroblast-specific splice variant of prolyl-4-hydroxylase, respectively. After correcting for fibroblast-associated CMV, the mean CMV load was reduced in whole blood by LD from 7.42 x 10(2) to 1.13 copies per microliter (2.81(10)log reduction) and from 3.8 x 10(2) to 4.77 copies per microliter (1.9(10)log reduction) in platelets. These results suggest that LD by filtration reduces viral burden but does not completely remove CMV from blood components.
Authors:
Micaela Rios Visconti; Joanne Pennington; Stephen F Garner; Jean-Pierre Allain; Lorna M Williamson
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-10-02
Journal Detail:
Title:  Blood     Volume:  103     ISSN:  0006-4971     ISO Abbreviation:  Blood     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-01-19     Completed Date:  2004-03-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1137-9     Citation Subset:  AIM; IM    
Affiliation:
Division of Transfusion Medicine, National Blood Service, Long Rd, Cambridge CB2 2PT, England.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Blood Component Removal / methods*
Blood Transfusion / adverse effects
Carrier State / blood,  virology
Coculture Techniques
Cytomegalovirus / genetics*,  isolation & purification*
Cytomegalovirus Infections / blood,  transmission,  virology
DNA, Viral / genetics
Fibroblasts / virology
Filtration / methods
Humans
Leukocytes, Mononuclear / virology
Lymphocyte Depletion / methods
RNA, Messenger / blood,  genetics
RNA, Viral / blood,  genetics
Reverse Transcriptase Polymerase Chain Reaction / methods*
Chemical
Reg. No./Substance:
0/DNA, Viral; 0/RNA, Messenger; 0/RNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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