Document Detail


Assessment of the predictive value of follicular fluid insulin, leptin and adiponectin in assisted reproductive cycles.
MedLine Citation:
PMID:  20170349     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To assess the correlation of intrafollicular insulin, leptin and adiponectin levels with assisted reproductive technologies (ART) outcome. METHODS: This was a retrospective study of 46 patients undergoing in vitro fertilisation/intracytoplasmic sperm injection. Follicular fluid (FF) samples collected at oocyte retrieval were assayed for insulin, leptin and adiponectin levels using enzyme-linked immunosorbent assay, and correlations with ART outcome were analysed. RESULTS: There was no significant correlation between intrafollicular insulin, leptin and adiponectin levels. There was a significant difference in the concentration of insulin (P = 0.007), but not leptin or adiponectin, between pregnant (n = 20) and non-pregnant (n = 26) cycles. Only two pregnancies was observed in the 12 cycles in which the concentration of insulin was greater than 7 mU/l in FF, while 18 pregnancies was observed in the 34 cycles in which the concentration of insulin was less than 7 mU/l (P = 0.043). The significantly high concentration of insulin in FF was observed in non-pregnant cycles of patients with polycystic ovary syndrome (PCOS). CONCLUSIONS: Our results suggest the possible involvement of intrafollicular insulin in folliculogenesis. Insulin resistance-related substances may affect the reproductive process in patients with PCOS.
Authors:
Sachiko Takikawa; Akira Iwase; Maki Goto; Toko Harata; Tomokazu Umezu; Tatsuo Nakahara; Hiroharu Kobayashi; Kyosuke Suzuki; Shuichi Manabe; Fumitaka Kikkawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology     Volume:  26     ISSN:  1473-0766     ISO Abbreviation:  Gynecol. Endocrinol.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-06-14     Completed Date:  2010-09-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8807913     Medline TA:  Gynecol Endocrinol     Country:  England    
Other Details:
Languages:  eng     Pagination:  494-9     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Showa-ku, Nagoya, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adiponectin / analysis*
Adult
Enzyme-Linked Immunosorbent Assay
Female
Follicular Fluid / chemistry*
Humans
Insulin / analysis*
Leptin / analysis*
Pregnancy
Pregnancy Outcome
Reproductive Techniques, Assisted*
Retrospective Studies
Statistics, Nonparametric
Chemical
Reg. No./Substance:
0/Adiponectin; 0/Leptin; 11061-68-0/Insulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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