Document Detail

Assessment of a practical method for identifying anovular dairy cows synchronized for first postpartum timed artificial insemination.
MedLine Citation:
PMID:  17582109     Owner:  NLM     Status:  MEDLINE    
Our objective was to determine whether a single examination of ovaries using transrectal ultrasonography at the first GnRH injection of a Presynch + Ovsynch protocol is a useful method for assessing cyclicity status and thereby enabling differential management of anovular vs. cyclic cows. Lactating Holstein cows (n = 842) receiving a Presynch + Ovsynch protocol to initiate first postpartum timed artificial insemination (TAI) were used to compare 2 methods for assessing cyclicity status before TAI. For the standard method (using RIA), blood samples were collected at the second PGF2alpha injection of Presynch and the first GnRH injection of Ovsynch, and cows with serum progesterone > or = 1.0 ng/mL in one or both samples were classified as cycling, whereas cows with serum progesterone < 1.0 ng/mL in both samples were classified as anovular. For the practical method, transrectal ultrasonography (U/S) was used to determine the presence or absence of a corpus luteum (CL) at the first GnRH injection of Ovsynch, and cows without CL were classified as anovular, whereas cows with CL were classified as cycling. Statistical agreement (kappa) between the RIA and U/S methods to identify cycling cows was 0.66. Sensitivity, specificity, positive predictive value, and negative predictive value of U/S to identify anovular status were 85.7, 87.7, 64.7, and 95.9%, respectively. We conclude that assessing the presence or absence of CL at the first GnRH injection of a Presynch + Ovsynch protocol using U/S is a reliable and practical method for identifying the cyclicity status of cows before first TAI, but may slightly overestimate the proportion of anovular cows compared with the RIA method.
E Silva; R A Sterry; P M Fricke
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Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Journal of dairy science     Volume:  90     ISSN:  1525-3198     ISO Abbreviation:  J. Dairy Sci.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-06-21     Completed Date:  2007-11-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985126R     Medline TA:  J Dairy Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3255-62     Citation Subset:  IM    
Department of Dairy Science, University of Wisconsin-Madison, Madison 53706, USA.
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MeSH Terms
Anovulation / ultrasonography,  veterinary*
Cattle / physiology*
Dairying / methods*
Estrus Synchronization / methods
Gonadotropin-Releasing Hormone / administration & dosage
Insemination, Artificial / methods,  veterinary*
Ovary / ultrasonography
Postpartum Period
Predictive Value of Tests
Sensitivity and Specificity
Reg. No./Substance:
33515-09-2/Gonadotropin-Releasing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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