Document Detail


Assessment of the potentiality of TDZ on multiple shoot induction in Bauhinia tomentosa L., A woody legume.
MedLine Citation:
PMID:  23134604     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
An efficient and reproducible protocol for in vitro multiplication of Bauhinia tomentosa L. was developed. Multiple shoots were regenerated from cotyledonary node and stem nodal segments excised from in vitro raised seedlings on Murashige and Skoog (MS) medium supplemented with different concentrations (0.1, 0.3, 0.5, 0.8 and 1.0 μM) of thidiazuron (TDZ). The maximum response (62.6%) was recorded on MS medium amended with 0.8 μM TDZ. A long exposure to TDZ for 8 weeks showed abnormalities such as fasciation and compact shoots formation. To avoid adverse effects of prolonged exposure to TDZ in long-term establishment, the culture were transferred to TDZ free MS medium for further multiplication and elongation. The highest number of shoots and shoot length were recorded at the end of fourth subculture passage. Ex vitro rooting was achieved when the basal cut end of regenerated shoots were dipped in 200 μM indole-3-butyric acid (IBA) for half an hour followed by their transplantation in plastic pots filled with sterile Soilrite™ where 60% plantlets grew well and all expressed normal development.
Authors:
Ruphi Naz; M Anis; I M Aref
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta biologica Hungarica     Volume:  63     ISSN:  0236-5383     ISO Abbreviation:  Acta. Biol. Hung.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8404358     Medline TA:  Acta Biol Hung     Country:  Hungary    
Other Details:
Languages:  eng     Pagination:  474-82     Citation Subset:  IM    
Affiliation:
Aligarh Muslim University Plant Biotechnology Laboratory, Department of Botany Aligarh 202 002 India King Saud University Department of Plant Production, College of Food & Agricultural Sciences P.O. Box 2460 Riyadh 11451 Saudi Arabia.
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