Document Detail

Assessment of the potentiality of TDZ on multiple shoot induction in Bauhinia tomentosa L., A woody legume.
MedLine Citation:
PMID:  23134604     Owner:  NLM     Status:  In-Data-Review    
An efficient and reproducible protocol for in vitro multiplication of Bauhinia tomentosa L. was developed. Multiple shoots were regenerated from cotyledonary node and stem nodal segments excised from in vitro raised seedlings on Murashige and Skoog (MS) medium supplemented with different concentrations (0.1, 0.3, 0.5, 0.8 and 1.0 μM) of thidiazuron (TDZ). The maximum response (62.6%) was recorded on MS medium amended with 0.8 μM TDZ. A long exposure to TDZ for 8 weeks showed abnormalities such as fasciation and compact shoots formation. To avoid adverse effects of prolonged exposure to TDZ in long-term establishment, the culture were transferred to TDZ free MS medium for further multiplication and elongation. The highest number of shoots and shoot length were recorded at the end of fourth subculture passage. Ex vitro rooting was achieved when the basal cut end of regenerated shoots were dipped in 200 μM indole-3-butyric acid (IBA) for half an hour followed by their transplantation in plastic pots filled with sterile Soilrite™ where 60% plantlets grew well and all expressed normal development.
Ruphi Naz; M Anis; I M Aref
Related Documents :
7936024 - Effects of low density lipoprotein on type iv collagen production by cultured rat mesan...
7454134 - Effects of hdl subclasses on 3-hydroxy-3-methylglutaryl coenzyme a reductase in human f...
12692004 - Low density lipoproteins interact with acidic fibroblast growth factor and modify its f...
16199524 - Dual roles for cholesterol in mammalian cells.
3465774 - In vivo and in vitro study of the effects of vitamin a deficiency on rat third molar de...
7430694 - Corticoids and human skin fibroblasts: intracellular specific binding in relation to gr...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta biologica Hungarica     Volume:  63     ISSN:  0236-5383     ISO Abbreviation:  Acta. Biol. Hung.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8404358     Medline TA:  Acta Biol Hung     Country:  Hungary    
Other Details:
Languages:  eng     Pagination:  474-82     Citation Subset:  IM    
Aligarh Muslim University Plant Biotechnology Laboratory, Department of Botany Aligarh 202 002 India King Saud University Department of Plant Production, College of Food & Agricultural Sciences P.O. Box 2460 Riyadh 11451 Saudi Arabia.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Development of chloroplast microsatellite markers for phylogenetic analysis in Brassicaceae.
Next Document:  DNA content alterations in Tetrahymena pyriformis macronucleus after exposure to food preservatives ...