| Assessment of poly(methacrylic acid-co-N-vinyl pyrrolidone) as a carrier for the oral delivery of therapeutic proteins using Caco-2 and HT29-MTX cell lines. | |
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MedLine Citation:
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PMID: 19213059 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hydrogels of poly(methacrylic acid-co-N-vinyl pyrrolidone) were synthesized and evaluated for their use as carriers for oral protein delivery. Insulin loading efficiencies were determined to be near 90% for carriers crosslinked with ethylene glycol dimethacrylate with corresponding weight incorporation levels near 12%. Although no insulin was released in gastric conditions, as desired, near instantaneous release occurred when the pH was raised to values typical of the intestinal area. Cytocompatibility studies with Caco-2 and Caco-2/HT29-MTX cultures demonstrated that microparticles did not elicit toxic effects at concentrations up to 5.0 mg/mL. Insulin transport studies revealed that the carriers did not disrupt the cell layer and thus did not change the insulin permeability in the apical-to-basolateral direction. Therefore, microparticles of this system were best suited for oral delivery of therapeutic agents that do not require transport facilitation. |
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Authors:
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Daniel A Carr; Nicholas A Peppas |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Journal of biomedical materials research. Part A Volume: 92 ISSN: 1552-4965 ISO Abbreviation: J Biomed Mater Res A Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2009-12-23 Completed Date: 2010-03-19 Revised Date: 2012-01-11 |
Medline Journal Info:
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Nlm Unique ID: 101234237 Medline TA: J Biomed Mater Res A Country: United States |
Other Details:
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Languages: eng Pagination: 504-12 Citation Subset: IM |
Copyright Information:
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(c) 2009 Wiley Periodicals, Inc. |
Affiliation:
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Department of Chemical Engineering, The University of Texas at Austin, 1 University Station C0400, Austin, Texas 78712, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Administration, Oral Algorithms Caco-2 Cells Cell Survival / drug effects Drug Carriers Drug Delivery Systems HT29 Cells Humans Hydrogels Hypoglycemic Agents / administration & dosage, chemistry Insulin / administration & dosage, chemistry Intestines / metabolism Kinetics Materials Testing Methylmethacrylates / chemistry* Nanoparticles Povidone / chemistry* Proteins / administration & dosage* Stomach / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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EB-000246/EB/NIBIB NIH HHS; R01 EB000246-15/EB/NIBIB NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Drug Carriers; 0/Hydrogels; 0/Hypoglycemic Agents; 0/Insulin; 0/Methylmethacrylates; 0/Proteins; 25655-01-0/poly(methylmethacrylate-N-vinylpyrrolidone); 9003-39-8/Povidone |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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