Document Detail


Assessment of photosensitizer dosimetry and tissue damage assay for photodynamic therapy in advanced-stage tumors.
MedLine Citation:
PMID:  15291303     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Photodynamic therapy (PDT) efficacy is a complex function of tissue sensitivity, photosensitizer (PS) uptake, tissue oxygen concentration, delivered light dose and some other parameters. To better understand the mechanisms and optimization of PDT treatment, we assessed two techniques for quantifying tissue PS concentration and two methods for quantifying pathological tumor damage. The two methods used to determine tissue PS concentration kinetic were in vivo fluorescence probe and ex vivo chemical extraction. Both methods show that the highest tumor to normal tissue PS uptake ratio appears 4 h after PS administration. Two different histopathologic techniques were used to quantify tumor and normal tissue damage. A planimetry assessment of regional tumor necrosis demonstrated a linear relationship with increasing light dose. However, in large murine tumors this finding was complicated by the presence of significant spontaneous necrosis. A second method (densitometry) assessed cell death by nuclear size and density. With some exceptions the densitometry method generally supported the planimetry results. Although the densitometry method is potentially more accurate, it has greater potential subjectivity. Finally, our research suggests that the tools or methods we are studying for quantifying PS levels and tissue damage are necessary for the understanding of PDT effect and therapeutic ratio in experimental in vivo tumor research.
Authors:
Chao Sheng; Brian W Pogue; Eileen Wang; John E Hutchins; P Jack Hoopes
Related Documents :
24182453 - Cytoplasmic oct4 staining is a sensitive marker of neuroendocrine differentiation.
24624323 - Desmoplastic small round cell tumor of the ovary: a rare but devastating disease in you...
2263613 - Analysis of interleukin 2 and various effector cell populations in adoptive immunothera...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Photochemistry and photobiology     Volume:  79     ISSN:  0031-8655     ISO Abbreviation:  Photochem. Photobiol.     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-08-04     Completed Date:  2004-09-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376425     Medline TA:  Photochem Photobiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  520-5     Citation Subset:  IM    
Affiliation:
Thayer School of Engineering, Dartmouth College, Hanover, NH, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Dose-Response Relationship, Radiation
Female
Light
Mammary Neoplasms, Experimental / drug therapy*,  metabolism,  pathology*
Mice
Mice, Inbred C3H
Necrosis
Organometallic Compounds / administration & dosage,  pharmacokinetics,  therapeutic use
Photochemotherapy / adverse effects*
Photosensitizing Agents / administration & dosage,  adverse effects*,  pharmacokinetics*,  therapeutic use
Tissue Distribution
Grant Support
ID/Acronym/Agency:
P01CA84203/CA/NCI NIH HHS; R01CA78734/CA/NCI NIH HHS; R44CA076913/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Organometallic Compounds; 0/Photosensitizing Agents; 0/chloroaluminum disulfonated phthalocyanine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A monochromatic action spectrum for the photoinduction of the UV-absorbing mycosporine-like amino ac...
Next Document:  Cooperative effects in the photophysical properties of self-associated triguanosine diphosphates.