Document Detail


Assessment of oxidative stress in lymphocytes with exercise.
MedLine Citation:
PMID:  21493722     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study investigated whether changes in the cellular composition of blood during exercise could partly account for observations of exercise-induced changes in lymphocyte oxidative stress markers. Markers of oxidative stress were assessed before and after 60 min of intense treadmill running. Samples were collected from 16 men (means ± SD: age 33 ± 13 yr; body mass index 23.8 ± 2.5 kg/m(2); maximal oxygen uptake 59.7 ± 5.2 ml·kg(-1)·min(-1)). Peripheral blood lymphocytes were assayed for protein carbonyl concentration, and plasma was assessed for lipid peroxides and antioxidant capacity. In a separate study, intracellular thiol concentration was determined in lymphocyte subsets from eight characteristically similar men by flow cytometry, of which T-cell memory populations were further identified on the basis of CD27, CD28, and CD45RA expression. Total lymphocyte protein carbonyls were transiently increased with exercise and returned to baseline within 15 min (P < 0.001). This change was accompanied by an increase in plasma lipid peroxides (P < 0.05) and total antioxidant capacity (P < 0.001). Correlation analyses showed that lymphocyte protein carbonyl content was not related to changes in the cellular composition of peripheral blood during exercise. Natural killer cells (CD3(-)CD56(+)) and late-differentiated/effector memory cells (CD4(+)/CD8(+)CD27(-)CD28(-)/CD45RA(+)), which mobilized most with exercise, showed high intracellular thiol content (P < 0.001). High thiol content suggests a lower oxidative load carried by these lymphocytes. Thus vigorous exercise resulted in a transient increase in lymphocyte oxidative stress. Results suggest this was unrelated to the alterations in the cellular composition of peripheral blood.
Authors:
James E Turner; Jos A Bosch; Mark T Drayson; Sarah Aldred
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-04-14
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  111     ISSN:  1522-1601     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-07-12     Completed Date:  2013-07-08     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  206-11     Citation Subset:  IM    
Affiliation:
School of Sport and Exercise Sciences, The University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
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MeSH Terms
Descriptor/Qualifier:
Adult
Analysis of Variance
Antigens, CD27 / blood
Antigens, CD28 / blood
Antigens, CD45 / blood
Biological Markers / blood
Exercise*
Exercise Test
Flow Cytometry
Glutathione / blood
Humans
Immunologic Memory
Immunophenotyping
Lipid Peroxidation
Lymphocytes / immunology,  metabolism*
Male
Middle Aged
Oxidative Stress*
Protein Carbonylation
Running
Sulfhydryl Compounds / blood
T-Lymphocyte Subsets / immunology,  metabolism
Time Factors
Young Adult
Chemical
Reg. No./Substance:
0/Antigens, CD27; 0/Antigens, CD28; 0/Biological Markers; 0/Sulfhydryl Compounds; 70-18-8/Glutathione; EC 3.1.3.48/Antigens, CD45

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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