| Assessment of oxidative stress in lymphocytes with exercise. | |
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MedLine Citation:
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PMID: 21493722 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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This study investigated whether changes in the cellular composition of blood during exercise, could partly account for observations of exercise-induced changes in lymphocyte oxidative stress markers. Markers of oxidative stress were assessed before and after 60 minutes of intense treadmill running. Samples were collected from sixteen men (mean ± SD; age 33 ± 13 y; body mass index 23.8 ± 2.5 kg(.)m(-2), maximal-oxygen uptake 59.7 ± 5.2 ml(.)kg(-1)(.)min(-1)). Peripheral blood lymphocytes were assayed for protein carbonyl concentration and plasma was assessed for lipid peroxides and antioxidant capacity. In a separate study, intracellular thiol concentration was determined in lymphocyte sub-sets from 8 characteristically similar men by flow cytometry, of which T cell memory populations were further identified on the basis of CD27, CD28, and CD45RA expression. Total lymphocyte protein carbonyls were transiently increased with exercise and returned to baseline within 15 minutes (p < .001). This change was accompanied by an increase in plasma lipid peroxides (p < .05) and total antioxidant capacity (p < .001). Correlation analyses showed that lymphocyte protein carbonyl content was not related to changes in the cellular composition of peripheral blood during exercise. Natural killer cells (CD3-CD56+) and late-differentiated/effector memory cells (CD4+/CD8+CD27-CD28-/CD45RA+) which mobilised most with exercise, showed high intracellular thiol content (p < .001). High thiol content suggests a lower oxidative load carried by these lymphocytes. Thus, vigorous exercise resulted in a transient increase in lymphocyte oxidative stress. Results suggest this was un-related to the alterations in the cellular composition of peripheral blood. |
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Authors:
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James E Turner; Jos A Bosch; Mark T Drayson; Sarah Aldred |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-4-14 |
Journal Detail:
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Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: - ISSN: 1522-1601 ISO Abbreviation: - Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-4-15 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1University of Birmingham. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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