| Assessment of neutrophil leukocyte secretory response to fMLP in whole blood in vitro. | |
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MedLine Citation:
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PMID: 1506768 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A simple, precise method has been developed for assessing neutrophil secretory responses (release of vitamin B12 binding protein from specific granules) to challenge of aliquots of whole blood with the bacterial chemotactic peptide N-formylmethionyl-leucyl-phenylalanine (fMLP). Dose-response studies performed on blood from normal healthy volunteers showed higher maximal secretory responses in males than females (33.3 +/- SEM 2.2 vs. 27.4 +/- 2.5, P less than .005) a left shift in dose-response curves after feeding compared to fasting (P less than .005), spontaneous up-regulation of responses in blood incubated at 37 degrees C for 1 h, and marked upregulation in response to preincubation with endotoxin. This whole blood challenge method may be used to study neutrophil responses in groups of individuals or patients without the confounding effects of changes in cell responses resulting from cell isolation procedures. The method may also be used as a bioassay for neutrophil-activating factors. |
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Authors:
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V S Chadwick; D M Ferry; T J Butt |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of leukocyte biology Volume: 52 ISSN: 0741-5400 ISO Abbreviation: J. Leukoc. Biol. Publication Date: 1992 Aug |
Date Detail:
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Created Date: 1992-09-22 Completed Date: 1992-09-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8405628 Medline TA: J Leukoc Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 143-50 Citation Subset: IM |
Affiliation:
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Department of Experimental Medicine, University of Otago Medical School, Dunedin, New Zealand. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cytochalasin B
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pharmacology Dose-Response Relationship, Drug Female Humans Male N-Formylmethionine Leucyl-Phenylalanine / pharmacology* Neutrophils / drug effects*, secretion Transcobalamins / secretion Up-Regulation |
| Chemical | |
Reg. No./Substance:
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0/Transcobalamins; 14930-96-2/Cytochalasin B; 59880-97-6/N-Formylmethionine Leucyl-Phenylalanine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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