Document Detail


Assessment of neurological injury due to circulatory arrest during profound hypothermia. An experimental study in vertebrates.
MedLine Citation:
PMID:  8217225     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To investigate brain changes in induced deep core hypothermia (15 degrees C) with circulatory arrest, five groups of neonatal pigs were subjected to cardiopulmonary bypass (CPB), with circulatory arrest (CA) periods varying from 70-120 min. The parameters analysed were: 1. Histology and electron microscopy of the brain six hours post-CPB, 2. Creatinophosphokinase (CPK) from cerebrospinal fluid (CSF), 3. Vasointestinal neuropeptide (VIP) and 7B2 specific neuropeptide both in plasma and brain tissue. The earliest morphological changes were seen after 90 min CA and were highly significant after 120 min arrest. These changes involved mainly the Purkinje cells of the interior half of the cerebellum with vacuolation in their cytoplasm. A rise in CPK in CSF occurred in all piglet-groups. The differences among the various groups were highly significant at 2 and 5 h post-CPB. (P < 0.05). Statistically significant differences were not exhibited among the various groups both in serum and brain tissue total mean values of VIP and 7B2 neuropeptides. We suggest that 1. The cerebellar region is the most sensitive where ischemic lesions attain their maximal severity and extent; the frequency and pattern of selective vulnerability of the cerebellum may be related primarily to its pattern of blood supply 2. The maximum time of CA without histopathological sequelae should not exceed 70 min.
Authors:
I T Fessatidis; V L Thomas; D F Shore; R H Hunt; R O Weller; F Goodland; D Rowe; M V Venetikou; S R Bloom
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery     Volume:  7     ISSN:  1010-7940     ISO Abbreviation:  Eur J Cardiothorac Surg     Publication Date:  1993  
Date Detail:
Created Date:  1993-12-08     Completed Date:  1993-12-08     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8804069     Medline TA:  Eur J Cardiothorac Surg     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  465-72; discussion 473     Citation Subset:  IM    
Affiliation:
Department of Cardiothoracic Surgery, Southampton University General Hospital, UK.
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MeSH Terms
Descriptor/Qualifier:
Anesthesia, General
Animals
Brain / pathology*
Brain Damage, Chronic / pathology*
Creatine Kinase / metabolism
Heart Arrest, Induced / adverse effects*
Hypothermia, Induced / adverse effects*
Hypoxia, Brain / pathology
Microscopy, Electron
Neuropeptides / metabolism
Swine
Thoracotomy
Vasoactive Intestinal Peptide / metabolism
Chemical
Reg. No./Substance:
0/Neuropeptides; 37221-79-7/Vasoactive Intestinal Peptide; EC 2.7.3.2/Creatine Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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