Document Detail

Assessment of myocardial oxidative stress in patients after myocardial revascularization.
MedLine Citation:
PMID:  3341166     Owner:  NLM     Status:  MEDLINE    
A homogeneous group of six patients, who underwent coronary artery bypass surgery, was studied to determine the presence of oxidative stress caused by oxygen-derived free radicals and its relationship with reperfusion cell damage. Biopsies were performed before ischemia and 10 minutes after reperfusion. The samples were assayed for hydroperoxide-initiated chemiluminescence and histochemical succinic dehydrogenase activity; the specimens were also studied by electron microscopy. The preischemic biopsy specimens showed chemiluminescence of 40 +/- 2 (cpm/mg protein) x 10(3), normal succinic dehydrogenase activity (grade 4), and generally preserved ultrastructure (necrotic/normal cells 5/100). However, the reperfusion biopsy specimens showed an increase in chemiluminescence to 91 +/- 19 (cpm/mg protein) x 10(3) (p less than 0.025), a partial loss of enzymatic activity (grade 2.6), and ultrastructural changes characterized by mitochondrial swelling and focal myofibrillar disorganization (necrotic/normal cells: 15/100; p less than 0.001). These observations seem to indicate the presence of oxidative stress during reoxygenation, a situation that may play a major role in the genesis of reperfusion injury. It appears to be the first observation relating free radical-induced oxidative stress to reperfusion injury in humans.
R Ferreira; S Llesuy; J Milei; D Scordo; H Hourquebie; L Molteni; C de Palma; A Boveris
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American heart journal     Volume:  115     ISSN:  0002-8703     ISO Abbreviation:  Am. Heart J.     Publication Date:  1988 Feb 
Date Detail:
Created Date:  1988-03-07     Completed Date:  1988-03-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370465     Medline TA:  Am Heart J     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  307-12     Citation Subset:  AIM; IM    
Section of Pathology, Institute of Cardiology, Buenos Aires, Argentina.
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MeSH Terms
Coronary Circulation
Free Radicals
Luminescent Measurements
Microscopy, Electron
Middle Aged
Mitochondria, Heart / ultrastructure
Mitochondrial Swelling
Myocardial Revascularization*
Myocardium / metabolism*,  ultrastructure
Succinate Dehydrogenase / metabolism*
Reg. No./Substance:
0/Free Radicals; EC Dehydrogenase

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