Document Detail


Assessment of mouse anxiety-like behaviour in the light-dark box and open-field arena: Role of equipment and procedure.
MedLine Citation:
PMID:  24832050     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Light-dark box and open field are conventional tests for assessment of anxiety-like behavior in the laboratory mice, based on approach-avoidance conflict. However, except the basic principles, variations in the equipment and procedures are very common. Therefore, contribution of certain methodological issues in different settings was investigated. Three inbred strains (C57BL/6, 129/Sv, DBA/2) and one outbred stock (ICR) of mice were used in the experiments. An effect of initial placement of mice either in the light or dark compartment was studied in the light-dark test. Moreover, two tracking systems were applied- position of the animals was detected either by infrared sensors in square box (1/2 dark) or by videotracking in rectangular box (1/3 dark). Both approaches revealed robust and consistent strain differences in the exploratory behaviour. In general, C57BL/6 and ICR mice showed reduced anxiety-like behaviour as compared to 129/Sv and DBA/2 strains. However, the latter two strains differed markedly in their behaviour. DBA/2 mice displayed high avoidance of the light compartment accompanied by thigmotaxis, whereas the hypoactive 129 mice spent a significant proportion of time in risk-assessment behaviour at the opening between two compartments. Starting from the light side increased the time spent in the light compartment and reduced the latency to the first transition. In the open field arena, black floor promoted exploratory behaviour - increased time and distance in the center and increased rearing compared to white floor. In conclusion, modifications of the apparatus and procedure had significant effects on approach-avoidance behaviour in general whereas the strain rankings remained unaffected.
Authors:
Natalia Kulesskaya; Vootele Voikar
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-5-12
Journal Detail:
Title:  Physiology & behavior     Volume:  -     ISSN:  1873-507X     ISO Abbreviation:  Physiol. Behav.     Publication Date:  2014 May 
Date Detail:
Created Date:  2014-5-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0151504     Medline TA:  Physiol Behav     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014. Published by Elsevier Inc.
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