Document Detail


Assessment of microcirculatory influence on cellular morphology in human burn wound healing using reflectance-mode-confocal microscopy.
MedLine Citation:
PMID:  19614915     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previous studies have assessed the effects of changes in microcirculation on wound healing; however, the influence of microcirculation on tissue histomorphology remains widely unknown. Reflectance-mode-confocal microscopy (RMCM) enables in vivo tissue observation on a cellular level. We present RMCM data evaluating the local microcirculation and assess the influence on histomorphology during burn healing. RMCM was performed in 12 patients (aged; 36.2+/-14.2 years, maximum-burn-extent: 4% total body surface area) at times 12, 36, and 72 hours after a superficial burn. The following parameters were assessed: quantitative blood-cell-flow (cbf), epidermal thickness (Emin), basal-layer thickness (tbl), and granular cell-size (Agran). Cbf was found to be 54+/-3.6 cells/minutes (control), increased to 91+/-3.6 cells/minutes (p<0.05) 12 hours postburn; decreased to 71+/-6.1 cells/minutes (p<0.05) (36 hours), and to 63+/-2.3 cells/minutes (p>0.05) 72 hours postburn. Emin was 43.74+/-3.87 mum (control), increased to 51.67+/-4.04 mum (p<0.05) 12 hours, decreased to 48.67+/-3.51 mum (p<0.05) 36 hours, and to 45.33+/-3.21 mum (p>0.05) at 72 hours postburn. Tbl was 14.17+/-0.6 mum (control), increased to 16.93+/-1.15 mum (p<0.05) 12 hours, decreased to 15.93+/-1.20 mum (p<0.05) 32 hours, and to 15.00+/-0.85 mum (p>0.05) 72 hours postburn. Agran was 718+/-56.20 mum(2) (control), increased to 901+/-66.02 mum(2) (p<0.05) 12 hours, decreased to 826+/-56.86 mum(2) 36 hours, and 766+/-65.06 mum(2) at 72 hours postburn. RMCM enables in vivo observation of wound microcirculation and allows direct assessment of vascular effects on cutaneous histomorphology during the healing course of superficial burns.
Authors:
Ahmet Ali Altintas; Mehmet Ali Altintas; Kyros Ipaktchi; Merlin Guggenheim; Panagiotis Theodorou; Pauangiotis Theodorou; Peymaneh Amini; Gerald Spilker
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society     Volume:  17     ISSN:  1524-475X     ISO Abbreviation:  Wound Repair Regen     Publication Date:    2009 Jul-Aug
Date Detail:
Created Date:  2009-07-20     Completed Date:  2009-09-24     Revised Date:  2009-12-23    
Medline Journal Info:
Nlm Unique ID:  9310939     Medline TA:  Wound Repair Regen     Country:  United States    
Other Details:
Languages:  eng     Pagination:  498-504     Citation Subset:  IM    
Affiliation:
Department of Hand, Plastic and Reconstructive Surgery, Burn Center Cologne, University of Witten Campus Cologne Merheim, 51109 Cologne, Germany. draltintas@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Adult
Burns / pathology*
Case-Control Studies
Dermis / blood supply*,  pathology*
Epidermis / pathology*,  physiopathology
Female
Humans
Image Processing, Computer-Assisted
Male
Microcirculation / physiology*
Microscopy, Confocal
Middle Aged
Wound Healing / physiology*
Young Adult
Comments/Corrections
Erratum In:
Wound Repair Regen. 2009 Sep-Oct;17(5):762
Note: Theodorou, Pauangiotis [corrected to Theodorou, Panagiotis]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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