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Assessment of methyl methacrylate genotoxicity by the micronucleus test.
MedLine Citation:
PMID:  23306624     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The aim of this study was to evaluate the genotoxic potential of methyl methacrylate (MMA) vapor by simulating standard occupational exposure of 8 hours per day and using the micronucleus test. We used 32 adult male Wistar rats divided into three groups: A - 16 rats exposed to MMA for 8 hours a day, B - Eight rats receiving single subcutaneous doses of cyclophosphamide on the first day of the experiment (positive control), C - Eight rats receiving only water and food ad libitum (negative control). Eight rats from group A and all of the rats from groups B and C were sacrificed 24 hours after beginning the experiment (acute exposure in group A). The remaining animals in group A were sacrificed 5 days after the experiment began (repeated exposure assessment in group A, simulating occupational exposure 40 hours/week). Femoral bone marrow was collected from each rat at the time of sacrifice for use in the micronucleus test. Two slides were completed per animal and were stained with Giemsa staining. Two thousand polychromatic erythrocytes were counted per animal. The Kruskal-Wallis test followed by a multiple comparisons test (Dunn test) was used for statistical analysis. The median number of micronuclei was 7.00 in the group exposed to MMA for 1 day, 2.00 in the group exposed to MMA for 5 days, 9.00 in the group exposed to cyclophosphamide (positive control) and 0.756 in the negative control group (p < 0.0001). MMA was genotoxic when measured after 1 day of exposure but was not evidently genotoxic after 5 days.
Authors:
Amarildo Mariano de Araújo; Guilherme Rodrigues Alves; Guilherme Trevisan Avanço; José Luiz Santos Parizi; Gisele Alborghetti Nai
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Brazilian oral research     Volume:  27     ISSN:  1807-3107     ISO Abbreviation:  Braz Oral Res     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101307187     Medline TA:  Braz Oral Res     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  31-6     Citation Subset:  D; IM    
Affiliation:
Univ do Oeste Paulista, Presidente Prudente, SP, Brazil.
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