| Assessment of liver steatosis and fibrosis in rats using integrated coherent anti-Stokes Raman scattering and multiphoton imaging technique. | |
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MedLine Citation:
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PMID: 22112129 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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We report the implementation of a unique integrated coherent anti-Stokes Raman scattering (CARS), second-harmonic generation (SHG), and two-photon excitation fluorescence (TPEF) microscopy imaging technique developed for label-free monitoring of the progression of liver steatosis and fibrosis generated in a bile duct ligation (BDL) rat model. Among the 21 adult rats used in this study, 18 rats were performed with BDL surgery and sacrificed each week from weeks 1 to 6 (n = 3 per week), respectively; whereas 3 rats as control were sacrificed at week 0. Colocalized imaging of the aggregated hepatic fats, collagen fibrils, and hepatocyte morphologies in liver tissue is realized by using the integrated CARS, SHG, and TPEF technique. The results show that there are significant accumulations of hepatic lipid droplets and collagen fibrils associated with severe hepatocyte necrosis in BDL rat liver as compared to a normal liver tissue. The volume of normal hepatocytes keeps decreasing and the fiber collagen content in BDL rat liver follows a growing trend until week 6; whereas the hepatic fat content reaches a maximum in week 4 and then appears to stop growing in week 6, indicating that liver steatosis and fibrosis induced in a BDL rat liver model may develop at different rates. This work demonstrates that the integrated CARS and multiphoton microscopy imaging technique has the potential to provide an effective means for early diagnosis and detection of liver steatosis and fibrosis without labeling. |
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Authors:
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Jian Lin; Fake Lu; Wei Zheng; Shuoyu Xu; Dean Tai; Hanry Yu; Zhiwei Huang |
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Publication Detail:
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Type: JOURNAL ARTICLE |
Journal Detail:
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Title: Journal of biomedical optics Volume: 16 ISSN: 1560-2281 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-11-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9605853 Medline TA: J Biomed Opt Country: - |
Other Details:
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Languages: ENG Pagination: 116024 Citation Subset: - |
Affiliation:
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National University of Singapore, Optical Bioimaging Laboratory, Department of Bioengineering, Faculty of Engineering, Singapore 117576Singapore-MIT Alliance, Computational and System Biology Program, Singapore 117576Institute of Bioengineering and Nanotechnology, Singapore 138669National University Health System, Yong Loo Lin School of Medicine, Department of Physiology, Singapore 117597Mechanobiology Institute, Singapore 117411Massachusetts Institute of Technology, Department of Biological Engineering, Cambridge, Massachusetts 02139. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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