Document Detail


Assessment of limbus and central cornea in patients with keratolimbal allograft transplantation using in vivo laser scanning confocal microscopy: an observational study.
MedLine Citation:
PMID:  21267594     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND: Keratolimbal allograft (KLAL) transplantation has been proved to be a useful surgical procedure for limbal stem cell deficiency patients. However, information about in vivo ocular surface changes in those patients is limited, due to the lack of a reliable and non-invasive technique for closely monitoring the changes of KLAL grafts. The aim of this study is to characterize the cellular changes in the limbus and central cornea after KLAL in patients with severe ocular chemical injury, using in vivo laser scanning confocal microscopy (LSCM). METHODS: This is a prospective, noncomparative, observational case series. Twenty-three patients (23 eyes) with total limbal stem cell deficiency due to ocular chemical injury were recruited. KLAL with or without other concurrent surgery were performed. LSCM and slit-lamp examination were performed on the limbus and the central cornea before surgery and at 6 and 12 months postoperatively. Presence of palisades of Vogt, limbal basal epithelial cell density within the palisades of Vogt (LEC), limbal dendritic cells (DC) density, and central corneal basal epithelial cell (CEC) density were assessed by LSCM. RESULTS: All patients completed 12 months of follow-up. Twenty-one patients were male and two were female, with a mean age of 39.5 ± 12.5 years. Six cases were due to acid burns, and the others were alkali burns. Palisades of Vogt were observed in all surviving grafts but were absent in graft failure. The epithelial cells in the central cornea of the failed graft had lost the classic polygonal morphology of the normal corneal basal epithelial cells. The cell density of LEC and CEC decreased significantly, whereas DC density increased in the failed grafts over time. CONCLUSIONS: In vivo LSCM is a useful tool for monitoring the cellular changes in KLAL grafts, and has the potential to diagnose the failure of KLAL grafts at the cellular level.
Authors:
Jiaxu Hong; Tianyu Zheng; Jianjiang Xu; Sophie X Deng; Ling Chen; Xinghuai Sun; Qihua Le; Yimin Li
Related Documents :
20384844 - Metabolic crossroads of iron and copper.
11048674 - Accumulation of protoporphyrin-ix (ppix) in leukemic cell lines following induction by ...
17574664 - In vitro effect of oligo-hydroxyalkanoates on the growth of mouse fibroblast cell line ...
16038594 - Intracellular sequestration of 223ra by the iron-storage protein ferritin.
15956554 - Extensive syncytium formation mediated by the reovirus fast proteins triggers apoptosis...
19595584 - Chemopreventive effect of cynodon dactylon (l.) pers. extract against dmh-induced colon...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-1-26
Journal Detail:
Title:  Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie     Volume:  -     ISSN:  1435-702X     ISO Abbreviation:  -     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-1-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8205248     Medline TA:  Graefes Arch Clin Exp Ophthalmol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Ophthalmology, Shanghai Eye, Ear, Nose and Throat Hospital, School of Shanghai Medicine, Fudan University, 83 Fenyang Road, Shanghai, 200031, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Two dominantly inherited ataxias linked to chromosome 16q22.1: SCA4 and SCA31 are not allelic.
Next Document:  Work-family conflict and prolonged fatigue among Japanese married male physicians.