Document Detail

Assessment of injury in transplanted and nontransplanted lungs after 6 h of cold storage with glutathione.
MedLine Citation:
PMID:  8005867     Owner:  NLM     Status:  MEDLINE    
Single-lung transplantation after 3 h of hypothermic storage produces bilateral lung injury [pulmonary reimplantation response (PRR)]. We hypothesized that glutathione (GSH) hypothermic storage would protect both lungs from PRR for extended preservation times and that differences in injury and protection would be realized between the graft and the nontransplanted lung. Mongrel dogs underwent left single-lung autotransplantation after preservation for 5-6 h in Euro-Collins (EC) solution, EC plus exogenous GSH (EC+GSH), or Viaspan (VIA) at 4 degrees C. Lung injury was measured in both lungs after 1 h of reperfusion. EC dogs demonstrated significant increases in lung edema, lipid peroxidation, and alveolar neutrophil recruitment in the lung graft and to a less extent in the nontransplanted right lung compared with control dogs (P < 0.05). Edema, lipid peroxidation, and alveolar neutrophils were significantly reduced in both lungs from EC+GSH and VIA dogs compared with lungs from EC dogs (P < 0.05). An increase in large-pore permeability was measured in the lung graft from EC dogs compared with all other lungs. Bronchoalveolar lavage fluid lactate dehydrogenase and total protein concentrations were elevated in both lungs from all three groups of tranplanted dogs compared with those of control dogs (P < 0.05). These data suggest that GSH-containing solutions attenuate the PRR after 6 h of ischemic hypothermic storage but that the protection is incomplete. Mechanisms of injury affecting the lung graft during the PRR appear to differ from those affecting the nontransplanted lung.
C L Bryan; A J Patefield; D Cohen; J L Nielsen; B Emanuel; J H Calhoon
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  76     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  1994 Mar 
Date Detail:
Created Date:  1994-07-15     Completed Date:  1994-07-15     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1232-41     Citation Subset:  IM    
Department of Medicine, University of Texas Health Science Center at San Antonio.
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MeSH Terms
Bronchoalveolar Lavage Fluid / cytology
Glutathione / metabolism,  pharmacology*
Hemodynamics / physiology
L-Lactate Dehydrogenase / metabolism
Lipid Peroxidation / physiology
Lung / pathology,  physiology
Lung Injury*
Lung Transplantation / physiology*
Organ Preservation*
Organ Size / physiology
Oxygen / blood
Proteins / metabolism
Pulmonary Circulation / physiology
Pulmonary Edema / pathology,  prevention & control
Thiobarbituric Acid Reactive Substances / metabolism
Reg. No./Substance:
0/Proteins; 0/Thiobarbituric Acid Reactive Substances; 70-18-8/Glutathione; 7782-44-7/Oxygen; EC Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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