Document Detail

Assessment of in vitro and in vivo recovery of sinomenine using microdialysis.
MedLine Citation:
PMID:  17610655     Owner:  NLM     Status:  MEDLINE    
BACKGROUND/PURPOSE: For microdialysis studies in the skin, laboratory-made linear probes are often used. The application of the microdialysis technique to the investigation of pharmacokinetics and pharmacodynamics of drugs requires careful assessment of the linear probes' performance to ensure validity of the data obtained using this technique. The aim of this study was to establish and validate the microdialysis technique for investigation of the pharmacokinetics and pharmacodynamics of sinomenine. METHODS: Using different lengths of the dialysis membrance and different perfusion flow rates, a flow rate of 2 microL/min with 20-min sampling intervals was selected for the subsequent studies, based on the analysis of sinomenine from the microdialysis probe. In vitro recovery of sinomenine from the microdialysis probe was independent of concentration, stable over an 8-h period. Comparable in vitro recoveries were obtained by different established approaches including recovery estimation by gain, loss and the zero-net flux (ZNF) method. Recovery by loss was used to study the in vivo recovery of sinomenine from rat subcutaneous tissue. RESULTS: The performance of the microdialysis system was stable over an 8-h study, resulting in a mean in vitro recovery of 51.91+/-1.29%. Recovery obtained using the ZNF plot was 52.43%. In vivo recovery of sinomenine was 34.46+/-0.76% and was stable over the 7-h study period. CONCLUSION: The in vitro and in vivo performance of the microdialysis technique was established for the study of sinomenine. It would prove to be a useful and reliable tool to study the pharmacokinetics and pharmacodynamics of sinomenine. The data obtained in our study highlight the importance of a systematic examination of microdialysis linear probe validation.
Hong Zheng; Li-Fu Shi; Jin-Hong Hu
Related Documents :
10332875 - Fitting cytosolic adp recovery after exercise with a step response function.
10644625 - Postcontractile force depression in humans is associated with an impairment in sr ca(2+...
12471305 - Clenbuterol diminishes aerobic performance in horses.
20949855 - The influence of exercise intensity on frontal electroencephalographic asymmetry and se...
468635 - Breath-by-breath variation of frc: effect on vo2 and vco2 measured at the mouth.
19544415 - Exercise increases neural stem cell number in a growth hormone-dependent manner, augmen...
Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)     Volume:  13     ISSN:  0909-752X     ISO Abbreviation:  Skin Res Technol     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-07-05     Completed Date:  2007-10-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9504453     Medline TA:  Skin Res Technol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  323-9     Citation Subset:  IM    
Department of Pharmacy, Changhai Hospital, Second Military Medical University, Shanghai, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Drug Industry / instrumentation*
Microdialysis / instrumentation,  methods*,  standards
Morphinans / chemistry,  pharmacokinetics*
Rats, Wistar
Reproducibility of Results
Skin / metabolism*
Reg. No./Substance:
0/Morphinans; 115-53-7/sinomenine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Fluorescent silica colloids for study and visualization of skin care products.
Next Document:  Optimization of the skin multiple analyte profile bioanalytical method for determination of skin bio...