Document Detail

Assessment of hepatobiliary function and placental barrier integrity in pregnant rats by combination of bromosulphophthalein and fluorescein isothiocyanate plasma disappearance tests.
MedLine Citation:
PMID:  7547429     Owner:  NLM     Status:  MEDLINE    
The permeability of the placental barrier to bromosulphophthalein (BSP) is believed to be very low. Whether this property is shared by other cholephilic organic anions, such as fluorescein isothiocyanate (FITC), is not known. When BSP was injected (140 mumol/kg body wt) into the left jugular vein of non-pregnant or pregnant female rats (at the 21st day of pregnancy), a similar and rapid plasma disappearance was observed during the first few minutes; afterwards, a slower disappearance phase was found. This phase was different in these groups, that is, a longer retention of BSP in the maternal bloodstream of pregnant rats was accompanied by a slower BSP output into bile. It was impossible to demonstrate the presence of BSP in fetal blood or the placenta by colorimetric methods. These results are consistent with the modifications occurring in the hepatic handling of BSP during pregnancy together with a marked impermeability of the placenta to the dye, at least in the mother-to-fetus direction. After intravenous FITC (10 mumol/kg body wt) administration to the mother, the compound was rapidly transferred into both the maternal bile and the fetal blood. Thereafter, FITC refluxed back from the fetal-placental compartment to the maternal blood as soon as the maternal liver reduced its plasma concentrations, which were first higher (approximately threefold) and then similar to those found in fetal blood. The reversible retention of FITC by the fetal-placental compartment accounts for a significant delay in both FITC bile output and plasma disappearance as compared with those observed in non-pregnant rats.(ABSTRACT TRUNCATED AT 250 WORDS)
G R Villaneuva; L I Sanchez-Abarca; P Bravo; M Y el-Mir; J J Marin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of experimental pathology     Volume:  76     ISSN:  0959-9673     ISO Abbreviation:  Int J Exp Pathol     Publication Date:  1995 Jun 
Date Detail:
Created Date:  1995-11-07     Completed Date:  1995-11-07     Revised Date:  2012-05-07    
Medline Journal Info:
Nlm Unique ID:  9014042     Medline TA:  Int J Exp Pathol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  183-90     Citation Subset:  IM    
Department of Physiology and Pharmacology, University of Salamanca, Spain.
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MeSH Terms
Bile / metabolism*
Fluorescein-5-isothiocyanate / diagnostic use*,  pharmacokinetics
Liver / metabolism
Liver Function Tests / methods*
Maternal-Fetal Exchange / physiology*
Placenta / metabolism
Placental Function Tests / methods*
Rats, Wistar
Sulfobromophthalein / diagnostic use*,  pharmacokinetics
Reg. No./Substance:
297-83-6/Sulfobromophthalein; 3326-32-7/Fluorescein-5-isothiocyanate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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