| Assessment of hepatic integrity after ischemic preservation by isolated perfusion in vitro: the role of albumin. | |
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MedLine Citation:
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PMID: 17324395 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Isolated perfusion of rat livers (IPRL) represents an attractive set-up to be used as a an evaluative tool in the easy and reproducible assessment of liver injury, allowing for screening of new approaches to organ preservation without the expenditure of actual transplantation experiments. Depending on the pathology under investigation, controversy exists concerning the inclusion of albumin in the IPRL. The present study evaluates the use of bovine serum albumin (BSA), simultaneously comparing its effect on healthy and ischemically challenged livers in the same model. Rat livers were excised, flushed via portal vein with Histidine-Tryptophan-Ketoglutarate (HTK) solution and preserved for up to 18 h in HTK at 4 degrees C. Perfusion was performed with Krebs-Henseleit buffer with or without addition of 3% BSA. Control preparations were perfused without prior ischemic storage. In the described model, stability of the preparations was documented for up to 120 min of isolated perfusion and addition of 3% BSA had no adverse effects on the viability of nonischemic livers. While liver perfusion without albumin was inappropriate to reveal alterations in parenchymal or vascular integrity after 18 h of cold preservation, albumin in the perfusate significantly and gradually unmasked differences between nonischemic liver preparations and livers stored ischemically for 8 or 18 h. It could be shown that BSA did have a significant modulatory effect on hepatic induction of apoptosis after ischemia in reducing cleavage of caspase 3. The implementation of albumin is advocated since experimental results are pivotally influenced by the presence or absence of this physiologically constitutive compound in the perfusate. |
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Authors:
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Thomas Minor; Steffen Manekeller |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-01-27 |
Journal Detail:
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Title: Cryobiology Volume: 54 ISSN: 0011-2240 ISO Abbreviation: Cryobiology Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2007-04-09 Completed Date: 2007-11-08 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0006252 Medline TA: Cryobiology Country: United States |
Other Details:
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Languages: eng Pagination: 188-95 Citation Subset: IM |
Affiliation:
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Surgical Research Division, University Clinic of Surgery, Bonn, Germany. chirfor@ukb.uni-bonn.de |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Albumins Animals Cold Temperature Liver* Male Organ Preservation / methods, standards* Perfusion / methods Rats Rats, Wistar Reperfusion Time Factors Tissue Survival Vascular Resistance |
| Chemical | |
Reg. No./Substance:
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0/Albumins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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