Document Detail


Assessment of the global two-stage method to EC50 determination.
MedLine Citation:
PMID:  9694169     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Preclinical studies of a new drug supply sets of concentration-effect observations. For each experiment, EC50 is estimated through fitting of the Emax model. Naive pooling of these individual estimates, also called the Standard Two-Stage method (STS), is usually performed. A better combination is obtained by the Global Two-Stage method (GTS) which takes into account the variability of the nonlinear regression estimation errors. The performances of STS and GTS are compared on real and simulated data. The results show that GTS performs better than STS in terms of bias or RMSE, especially in case of poorly designed experiments. Degradation of the quality of STS results in simulations appears to be mainly due to some experiments that are usually rejected by experimenters. Such rejections are avoided when using GTS, which hence is particularly suitable for systematic treatment of this kind of data.
Authors:
F Patron-Bizet; F Mentré; M Genton; C Thomas-Haimez; J Maccario
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of pharmacological and toxicological methods     Volume:  39     ISSN:  1056-8719     ISO Abbreviation:  J Pharmacol Toxicol Methods     Publication Date:  1998 Mar 
Date Detail:
Created Date:  1999-05-03     Completed Date:  1999-05-03     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  9206091     Medline TA:  J Pharmacol Toxicol Methods     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  103-8     Citation Subset:  IM    
Affiliation:
Department of Biostatistics, Institut de Recherches SERVIER, Suresnes, France.
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MeSH Terms
Descriptor/Qualifier:
Chemistry, Pharmaceutical / methods
Dose-Response Relationship, Drug
Drug Evaluation, Preclinical / methods*
Models, Statistical
Muscle Contraction / drug effects
Nonlinear Dynamics
Observer Variation
Saphenous Vein / drug effects

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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