Document Detail


Assessment of fetomaternal hemorrhage by flow cytometry and Kleihauer-Betke test in Rh-negative pregnancies.
MedLine Citation:
PMID:  17878734     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: To assess the efficacy of flow cytometry (FC) in the detection and quantification of fetomaternal hemorrhage (FMH) in comparison to the Kleihauer-Betke test (KBT). METHODS: 25 unsensitized Rh-negative mothers who had delivered Rh-positive infants were included. Presence of FMH was determined by KBT and FC using FITC-labeled BRAD-3 antibodies. RESULTS: FMH was detected in 19 (76%) patients by FC and 23 (92%) patients by KBT prior to delivery, and in 21 (84%) patients by FC and 23 (92%) patients by KBT after delivery. The mean volume of FMH in the post-delivery samples by KBT and FC were 0.34 +/- 0.26 ml (range 0.05-1.2 ml) and 0.37 +/- 0.57 ml (range 0.02-2.6 ml) respectively. The volume of post-delivery FMH estimated by KBT and FC correlated well (r = 0.75; ICC alpha = 0.73). A higher agreement between KBT and FC was seen in the 0.1-0.5 ml range (kappa = 0.65; p < 0.01). CONCLUSIONS: Both manual KBT and FC using FITC-BRAD-3 antibodies show good sensitivity in detecting and quantifying fetal red cells. There is a good correlation between the methods in the 0.1- to 0.5-ml range of FMH.
Authors:
S Savithrisowmya; Meenakshi Singh; Alka Kriplani; Nutan Agarwal; N K Mehra; Neerja Bhatla
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Publication Detail:
Type:  Journal Article     Date:  2007-09-18
Journal Detail:
Title:  Gynecologic and obstetric investigation     Volume:  65     ISSN:  1423-002X     ISO Abbreviation:  Gynecol. Obstet. Invest.     Publication Date:  2008  
Date Detail:
Created Date:  2008-03-05     Completed Date:  2008-04-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7900587     Medline TA:  Gynecol Obstet Invest     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  84-8     Citation Subset:  IM    
Copyright Information:
(c) 2008 S. Karger AG, Basel.
Affiliation:
Department of Obstetrics and Gynecology, All India Institute of Medical Sciences, New Delhi, India.
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MeSH Terms
Descriptor/Qualifier:
Adult
Antibodies, Monoclonal / diagnostic use*
Female
Fetomaternal Transfusion / blood,  diagnosis*
Flow Cytometry / methods*
Gestational Age
Humans
Infant, Newborn
Postpartum Period
Predictive Value of Tests
Pregnancy
Prenatal Diagnosis
Rh-Hr Blood-Group System / immunology*
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Rh-Hr Blood-Group System

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