Document Detail

Assessment of fetal-maternal haemorrhage in mothers with hereditary persistence of fetal haemoglobin.
MedLine Citation:
PMID:  1698825     Owner:  NLM     Status:  MEDLINE    
Kleihauer examination of peripheral blood cannot be used reliably to detect transplacental fetal-maternal haemorrhage in mothers with hereditary persistence of fetal haemoglobin (HPFH). In Rh(D) negative pregnancies diagnostic confusion with a large fetal-maternal haemorrhage could result in the administration of inappropriately excessive amounts of anti-D immunoglobulin, and the inability to diagnose and quantify transplacental haemorrhage in maternal HPFH by current methods could result in insufficient anti-D administration and subsequent Rh(D) sensitisation. Accordingly, a method to detect and quantify fetal-Rh(D) positive maternal haemorrhage using erythrocyte fluorescent immunocytometry was developed. An indirect immunofluorescence method with IgG anti-D immunoglobulin as the primary antibody was used, combined with quantitative analysis on a fluorescence activated cell sorter. The method was accurate, specific, and sensitive and could detect a contaminating population of 0.1% Rh(D) positive cells in Rh(D) negative blood--a level of fetal-maternal haemorrhage well covered by a single dose of 500 IU of anti-D immunoglobulin.
W N Patton; G S Nicholson; A H Sawers; I M Franklin; F A Ala; A W Simpson
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Publication Detail:
Type:  Case Reports; Journal Article    
Journal Detail:
Title:  Journal of clinical pathology     Volume:  43     ISSN:  0021-9746     ISO Abbreviation:  J. Clin. Pathol.     Publication Date:  1990 Sep 
Date Detail:
Created Date:  1990-11-09     Completed Date:  1990-11-09     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0376601     Medline TA:  J Clin Pathol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  728-31     Citation Subset:  AIM; IM    
Department of Haematology, Queen Elizabeth Hospital, Edgbaston.
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MeSH Terms
Cell Separation
Fetal Hemoglobin / analysis*
Fetomaternal Transfusion / diagnosis*
Flow Cytometry*
Hemoglobinopathies / genetics*
Immunoglobulin G / diagnostic use
Immunoglobulins / diagnostic use*
Isoantibodies / diagnostic use*
Peptides / blood
Rh-Hr Blood-Group System
Rho(D) Immune Globulin
Reg. No./Substance:
0/Immunoglobulin G; 0/Immunoglobulins; 0/Isoantibodies; 0/Peptides; 0/Rh-Hr Blood-Group System; 0/Rho(D) Immune Globulin; 0/Rho(D) antigen; 9034-63-3/Fetal Hemoglobin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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