Document Detail

Assessment of erythrocyte deformability by constant flow filtration technique: analysis of factors influencing the initial pressure.
MedLine Citation:
PMID:  6591985     Owner:  NLM     Status:  MEDLINE    
When filtering red blood cells (RBC) resuspended into buffer at constant flow, the pressure-time curve is dependent on erythrocyte deformability (E.D) and also on the plugging of pores by RBC or leukocytes (WBC). To understand the influence of these 3 factors, we used an apparatus with: 1) a constant flow syringe (Bioblock) (1.56 ml/mn); 2) a pressure transducer (Statham P23 ID); 3) a recorder (Gould); 4) a 5 micron polycarbonate filter (Nuclepore) housed in a lucite block according to USAMI & al. (1). Resuspensions of RBC from 4 to 14% haematocrit with 0 to 600 WBC/mm3 were investigated. Results indicate that: 1/ The first inflexion point of the pressure-time curve (initial pressure leading to calculation of beta) is related to the RBC's deformability; 2/beta is (in our experimental conditions) dependent on the WBC number; 3/beta seems very sensitive to deformability changes as shown by rapid increases induced by osmolarity changes.
M T Chan; E Catry; D Weill; G A Marcel; C George
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biorheology. Supplement : the official journal of the International Society of Biorheology     Volume:  1     ISSN:  0891-978X     ISO Abbreviation:  Biorheology Suppl     Publication Date:  1984  
Date Detail:
Created Date:  1984-11-09     Completed Date:  1984-11-09     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8503530     Medline TA:  Biorheology Suppl     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  267-70     Citation Subset:  IM    
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MeSH Terms
Erythrocytes / physiology*
Leukocytes / physiology
Osmolar Concentration
Ultrafiltration / methods

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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