Document Detail

Assessment of enamel erosion and protective effect of salivary pellicle by surface roughness analysis and scanning electron microscopy.
MedLine Citation:
PMID:  15641759     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To assess dental erosion caused by 0.1% and 1.0% citric acid in vitro and to estimate the protective influence of experimentally formed salivary pellicle. MATERIALS AND METHODS: Bovine enamel slabs (n = 80) were polished and embedded in epoxy resin. For the formation of pellicle layer 40 specimens were immersed for 24 h in pooled human saliva. Erosion was caused by immersion in citric acid solution for 1, 5, 10 and 30 min. Erosive alterations on the pellicle-covered and non-covered enamel specimens were scored as a change (delta) of surface roughness parameters Ra, Rt and RzDIN using contact profilometer and observed in scanning electron microscope. RESULTS: Profilometric analysis of eroded enamel specimens emphasized the aggressiveness of even low concentrated citric acid with a short period of challenge. The change of roughness parameters after 1-min immersion in 0.1% citric acid were 16.4, 182.6 and 132.2 nm for deltaRa, deltaRt and deltaRzDIN, respectively, and 54.8, 516.6 and 258.2 nm after 1-min immersion in 1.0% citric acid. Changes of the surface roughness were dependent on the exposure time and concentration of acidic solution. Pellicle layer significantly reduced the extent of erosive destruction, which was additionally documented on SEM-micrographs. Residual pellicle-like structures were detected after 5 min of immersion in 0.1% citric acid. However, there were no significant differences in pellicle-covered and non-covered enamel slabs measured profilometrically for 1.0% citric acid with 10 min and 30 min exposure time. CONCLUSION: The findings confirm the property of pellicle layer to resist against erosive influence of organic acids, which is, however, limited by duration of acidic treatment and concentration of erosive agent.
Yuriy Nekrashevych; Matthias Hannig; Lutz Stösser
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Oral health & preventive dentistry     Volume:  2     ISSN:  1602-1622     ISO Abbreviation:  Oral Health Prev Dent     Publication Date:  2004  
Date Detail:
Created Date:  2005-01-11     Completed Date:  2005-03-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101167768     Medline TA:  Oral Health Prev Dent     Country:  England    
Other Details:
Languages:  eng     Pagination:  5-11     Citation Subset:  D; IM    
Department of Periodontology and Conservative Dentistry, Saarland University, Homburg, Germany.
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MeSH Terms
Citric Acid
Dental Deposits / physiopathology,  ultrastructure*
Dental Enamel / physiology,  ultrastructure*
Dental Enamel Solubility / physiology
Dental Pellicle / physiology,  ultrastructure*
Random Allocation
Saliva / physiology*
Surface Properties
Tooth Demineralization / chemically induced
Tooth Erosion / chemically induced,  pathology*,  physiopathology
Reg. No./Substance:
77-92-9/Citric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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