| Assessment of developmental delay in the zebrafish embryo teratogenicity assay. | |
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MedLine Citation:
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PMID: 22898132 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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In this study we analyzed some aspects of the assessment of developmental delay in the zebrafish embryotoxicity/teratogenicity test and explored the suitability of acetylcholinesterase (AChE) activity as a biochemical marker and as a higher throughput alternative to morphological endpoints such as head-trunk angle, tail length and morphological score. Embryos were exposed from 4 to 52h post-fertilization (hpf) to a selection of known embryotoxic/teratogen compounds (valproic acid, retinoic acid, caffeine, sodium salicylate, glucose, hydroxyurea, methoxyacetic acid, boric acid and paraoxon-methyl) over a concentration range. They were evaluated for AChE activity, head-trunk angle, tail length and several qualitative parameters integrated in a morphological score. In general, the different patterns of the concentration-response curves allowed distinguishing between chemicals that produced growth retardation (valproic and methoxyacetic acid) and chemicals that produced non-growth-delay related malformations. An acceptable correlation between the morphological score, AChE activity and head-trunk angle as markers of developmental delay was observed, being AChE activity particularly sensitive to detect delay in the absence of malformations. |
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Authors:
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E Teixidó; E Piqué; J Gómez-Catalán; J M Llobet |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-8-8 |
Journal Detail:
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Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: - ISSN: 1879-3177 ISO Abbreviation: Toxicol In Vitro Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-8-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012. Published by Elsevier Ltd. |
Affiliation:
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GRET-CERETOX and Toxicology Unit, Public Health Department, School of Pharmacy, University of Barcelona. Av. Joan XXIII s/n, E-08028 Barcelona, Spain. |
Export Citation:
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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