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Assessment of the contribution of electron microscopy to nanoparticle characterization sampled with two cascade impactors.
MedLine Citation:
PMID:  23356435     Owner:  NLM     Status:  In-Data-Review    
This study assessed the contribution of electron microscopy to the characterization of nanoparticles and compared the degree of variability in sizes observed within each stage when sampled by two cascade impactors: an Electrical Low Pressure Impactor (ELPI) and a Micro-Orifice Uniform Deposit Impactor (MOUDI). A TiO(2) nanoparticle (5 nm) suspension was aerosolized in an inhalation chamber. Nanoparticles sampled by the impactors were collected on aluminum substrates or TEM carbon-coated copper grids using templates, specifically designed in our laboratories, for scanning and transmission electron microscopy (SEM, TEM) analysis, respectively. Nanoparticles were characterized using both SEM and TEM. Three different types of diameters (inner, outer, and circular) were measured by image analysis based on count and volume, for each impactor stage. Electron microscopy, especially TEM, is well suited for the characterization of nanoparticles. The MOUDI, probably because of the rotation of its collection stages, which can minimize the resuspension of particles, gave more stable results and smaller geometric standard deviations per stage. Our data suggest that the best approach to estimate particle size by electron microscopy would rely on geometric means of measured circular diameters. Overall, the most reliable data were provided by the MOUDI and the TEM sampling technique on carbon-coated copper grids for this specific experiment. This study indicates interesting findings related to the assessment of impactors combined with electron microscopy for nanoparticle characterization. For future research, since cascade impactors are extensively used to characterize nano-aerosol exposure scenarios, high-performance field emission scanning electron microscopy (FESEM) should also be considered.
Alexandra Noël; Gilles L'espérance; Yves Cloutier; Philippe Plamondon; Julie Boucher; Suzanne Philippe; Chantal Dion; Ginette Truchon; Joseph Zayed
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of occupational and environmental hygiene     Volume:  10     ISSN:  1545-9632     ISO Abbreviation:  J Occup Environ Hyg     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-01-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101189458     Medline TA:  J Occup Environ Hyg     Country:  England    
Other Details:
Languages:  eng     Pagination:  155-72     Citation Subset:  IM    
a Département de Santé environnementale et Santé au travail, Faculté de Médecine , Université de Montréal , Montréal , Québec , Canada.
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