Document Detail


Assessment of the contribution of NAD(P)H-dependent quinone oxidoreductase 1 (NQO1) to the reduction of vitamin K in wildtype and NQO1-deficient mice.
MedLine Citation:
PMID:  24015818     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
NQO1 (DT-diaphorase) is a cytosolic enzyme that catalyzes the two-electron reduction of various quinones including vitamin K. The enzyme may play a role in vitamin K metabolism by reducing vitamin K to vitamin K hydroquinone for utilization in the post-translational γ-glutamyl carboxylation reactions required by several proteins involved in blood coagulation. The aim of this study was to assess the contribution of NQO1 to vitamin K reduction and hemostasis in an in vivo model. We examined the contribution of NQO1 to hemostasis by examining survival rates in mice poisoned with the anticoagulant warfarin. Supraphysiological amounts of vitamin K sufficiently reversed the effects of warfarin in both wildtype and NQO1 deficient mice. Additionally, vitamin K reductase activities distinct from vitamin K epoxide reductase (VKOR) and NQO1 were measured in vitro from both wildtype and NQO1-defecient mice. Our results suggest that NQO1 does not play a major role in the production of vitamin K hydroquinone and supports the existence of multiple vitamin K reduction pathways. The properties of a NAD(P)H dependent vitamin K reductase different from NQO1 are described.
Authors:
Brian O Ingram; Jared L Turbyfill; Peggy J Bledsoe; Anil K Jaiswal; Darrel W Stafford
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-9-9
Journal Detail:
Title:  The Biochemical journal     Volume:  -     ISSN:  1470-8728     ISO Abbreviation:  Biochem. J.     Publication Date:  2013 Sep 
Date Detail:
Created Date:  2013-9-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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