Document Detail

Assessment of the colorimetric and fluorometric assays for alkaline phosphatase activity in cow's, goat's, and sheep's milk.
MedLine Citation:
PMID:  18810873     Owner:  NLM     Status:  MEDLINE    
Raw milk is a well-established vehicle for the carriage of human pathogens, and many regulatory bodies have consequently mandated compulsory pasteurization as a food safety intervention. The residual activity of alkaline phosphatase (ALP) has historically been used to verify the adequacy of pasteurization of cow's milk. However, there is uncertainty on how the current ALP standards and methods of analysis can be applied to sheep's and goat's milk, which naturally contain different levels of the enzyme than that found in cow's milk. The official ALP methods applied in Canada (colorimetric assay; MFO-3) and in the United States (Fluorophos) were assessed for their ability to detect enzyme activity in raw and pasteurized milk derived from cows, sheep, and goats. The detection limit and the limit of quantitation were 0.8 and 2.02 microg/ml phenol, respectively, for the MFO-3 method and 43 and 85 mU/liter, respectively, for the Fluorophos method. The average ALP levels in raw goat's, cow's, and sheep's milk were 165, 1,562, and 3,512 microg/ml phenol, respectively. Raw milk detection limits, which correspond to raw milk phosphatase levels, were 0.051, 0.485, and 0.023% in cow's, goat's, and sheep's milk, respectively, for the MFO-3 method and 0.007, 0.070, and 0.004%, respectively, for the Fluorophos method. Although both methods can be used for ALP determination in cow's, goat's, and sheep's milk, the Fluorophos assay was superior to the colorimetric MFO-3 method based on sensitivity and time required to complete the analysis.
V Klotz; Art Hill; K Warriner; M Griffiths; J Odumeru
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of food protection     Volume:  71     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-09-24     Completed Date:  2008-11-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1884-8     Citation Subset:  IM    
Laboratory Services Division, University of Guelph, Guelph, Ontario, Canada N1H 8J7.
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MeSH Terms
Alkaline Phosphatase / analysis,  metabolism*
Colorimetry / methods*
Consumer Product Safety
Fluorometry / methods*
Food Contamination / analysis*
Food Handling / methods,  standards*
Food-Processing Industry / standards
Milk / enzymology*
Reproducibility of Results
Sensitivity and Specificity
Species Specificity
Reg. No./Substance:
EC Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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