Document Detail


Assessment of the chemical changes induced in human melanoma cells by boric acid treatment using infrared imaging.
MedLine Citation:
PMID:  20448936     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Boron is found in everyday foods and drinking water in trace quantities. Boron exists as boric acid (BA) within plants and animals, where low levels have been linked to cancer incidence. However, this correlation is not well characterized. In this study, we examined the chemical and morphological effects of BA on human skin melanoma cells (SK-MEL28) using Fourier Transform InfraRed Imaging (FTIRI) with a Focal Plane Array (FPA) detector. Cells were grown under concentrations of BA ranging from 0 to 50 mM. Cell viability was determined after 1, 2, 3, 5, 7 and 10 days using trypan blue staining. With FTIRI, images of approximately twenty cells per time point per condition were collected. Principal components analysis (PCA) was used to evaluate changes in cell composition, with particular focus on the lipid, protein, and nucleic acid spectral components. Results from trypan blue staining revealed decreased cell viability as BA concentration increased. FTIRI data indicated that the protein and lipid contents (as indicated by the lipid/protein ratio) did not undergo substantial changes due to BA treatment. In contrast, the nucleic acid/protein ratio significantly decreased with BA treatment. PCA results showed an increase in beta-sheet protein at higher concentrations of BA (12.5, 25, and 50 mM). Together, these results suggest that high concentrations of BA have an anti-proliferative effect and show signs consistent with apoptosis.
Authors:
Alvin S Acerbo; Lisa M Miller
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-06-10
Journal Detail:
Title:  The Analyst     Volume:  134     ISSN:  1364-5528     ISO Abbreviation:  Analyst     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2010-05-07     Completed Date:  2010-08-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372652     Medline TA:  Analyst     Country:  England    
Other Details:
Languages:  eng     Pagination:  1669-74     Citation Subset:  IM    
Affiliation:
Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY 11794, USA.
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MeSH Terms
Descriptor/Qualifier:
Boric Acids / pharmacology*
Diagnostic Imaging
Humans
Infrared Rays
Melanoma / chemistry,  metabolism*
Nucleic Acids / analysis
Proteins / analysis
Skin Neoplasms / pathology*
Spectrophotometry, Infrared
Spectroscopy, Fourier Transform Infrared / methods*
Tissue Distribution / drug effects
Chemical
Reg. No./Substance:
0/Boric Acids; 0/Nucleic Acids; 0/Proteins; 11113-50-1/boric acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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