Document Detail

Assessment of cell sheets derived from human periodontal ligament cells: a pre-clinical study.
MedLine Citation:
PMID:  20632035     Owner:  NLM     Status:  MEDLINE    
Periodontal-ligament-derived cells (PDL cells) have stem-cell-like properties and, when implanted into periodontal defects in vivo, can induce periodontal regeneration including the formation of new bone, cementum, and periodontal ligament. We have previously demonstrated that PDL cell sheets, harvested from temperature-responsive cell culture dishes, have a great potential for periodontal regeneration. The purpose of this study has been to validate the safety and efficacy of human PDL (hPDL) cell sheets for use in clinical trials. hPDL tissues from three donors were enzymatically digested, and the obtained cells were cultured with media containing autologous serum in a cell-processing center (CPC). The safety and efficacy of hPDL cell sheets were evaluated both in vitro and in vivo. In vitro studies showed that the hPDL cell sheets had high alkaline phosphatase activity and periostin expression (known PDL markers) and no contamination with microorganisms. In vivo studies revealed that hPDL cell sheets, implanted with dentin blocks, induced the formation of cementum and PDL-like tissue in immunodeficient mice. The hPDL cells presented no evidence of malignant transformation. Thus, hPDL cell sheets created in CPCs are safe products and possess the potential to regenerate periodontal tissues.
Kaoru Washio; Takanori Iwata; Manabu Mizutani; Tomohiro Ando; Masayuki Yamato; Teruo Okano; Isao Ishikawa
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; Validation Studies     Date:  2010-07-15
Journal Detail:
Title:  Cell and tissue research     Volume:  341     ISSN:  1432-0878     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-09-08     Completed Date:  2011-01-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  397-404     Citation Subset:  IM    
Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.
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MeSH Terms
Cell Culture Techniques / standards
Cells, Cultured
Dentin / cytology,  metabolism,  physiology
Feasibility Studies
Guided Tissue Regeneration / instrumentation,  methods*,  standards
Mice, Inbred C57BL
Mice, Inbred ICR
Mice, SCID
Models, Biological
Periodontal Ligament / cytology*,  physiology*,  transplantation
Quality Control
Tissue Scaffolds* / standards

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