| Assessment of cell-free DNA with microvascular complication of type II diabetes mellitus, using PCR and ELISA. | |
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MedLine Citation:
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PMID: 20408053 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In order to assess the potential biochemical markers in the development, diagnosis, and prognosis of diabetic patient with microvascular complication represented with retinopathy, we analyzed the levels of cell-free DNA by two different techniques. The levels of cell-free GAPDH assayed by quantitative PCR were significantly higher in the plasma samples of diabetic patients with and without diabetic retinopathy than in those of the control group; thus, it is a better biomarker than nucleosomes assayed by ELISA in patients with type 2 diabetes for the early detection of development of microvascular complications as retinopathy. |
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Authors:
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Shereen A El Tarhouny; Khaled M Hadhoud; Magdy M Ebrahem; Nashwa M Al Azizi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Nucleosides, nucleotides & nucleic acids Volume: 29 ISSN: 1532-2335 ISO Abbreviation: Nucleosides Nucleotides Nucleic Acids Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-04-21 Completed Date: 2010-09-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100892832 Medline TA: Nucleosides Nucleotides Nucleic Acids Country: England |
Other Details:
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Languages: eng Pagination: 228-36 Citation Subset: IM |
Affiliation:
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Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt. shereeneltarhony@hotmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aged Cells DNA / blood, genetics* Diabetes Complications / blood, genetics* Diabetes Mellitus, Type 2 / blood, genetics* Enzyme-Linked Immunosorbent Assay Female Humans Male Middle Aged Nucleosomes / chemistry Reverse Transcriptase Polymerase Chain Reaction Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Nucleosomes; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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