| Assessment of the bioactivity of human and bovine thyrotrophin preparations using a porcine thyroid cell bioassay. | |
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MedLine Citation:
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PMID: 2764487 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The biopotency of six preparations of thyrotrophin (TSH) has been compared in a highly sensitive in vitro porcine thyroid cell bioassay using iodide uptake as an endpoint. Three of these preparations were of human origin and three derived from bovine pituitary tissue. One human TSH preparation, the 2nd International Reference Preparation, 80/558, was used to calibrate the other five. The log dose-log response curves for all preparations were sigmoidal in shape. For the purpose of evaluation the central linear portions of the curves were compared. With all preparations the slopes in this region were very similar. The relative biopotencies of the bovine preparations (unit:unit) were at least five times those of the human standards when measured using the porcine thyroid cell bioassay. These findings emphasise the need to control the TSH standards employed in a variety of bioassays, particularly those used for between-laboratory comparison. |
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Authors:
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S C Reader; B Davison; W R Robertson; A Lambert |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Annals of clinical biochemistry Volume: 26 ( Pt 4) ISSN: 0004-5632 ISO Abbreviation: Ann. Clin. Biochem. Publication Date: 1989 Jul |
Date Detail:
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Created Date: 1989-09-18 Completed Date: 1989-09-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0324055 Medline TA: Ann Clin Biochem Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 341-5 Citation Subset: IM |
Affiliation:
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Department of Chemical Pathology, Hope Hospital, Salford, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Assay Cattle Humans Iodides / metabolism Species Specificity Swine Thyroid Gland / cytology, metabolism Thyrotropin / analysis, standards* |
| Chemical | |
Reg. No./Substance:
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0/Iodides; 9002-71-5/Thyrotropin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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