Document Detail


Assessment of the benzethonium chloride method for routine determination of protein in cerebrospinal fluid and urine.
MedLine Citation:
PMID:  6821941     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have tested the characteristics of the method of Iwata and Nishikaze (Clin Chem 25: 1317, 1979). The linearity, sensitivity, and precision are satisfactory and the reactivity of benzethonium chloride with various proteins (albumin, immunoglobulins) is the same. The method has been compared with Meulemans's technique (Clin Chim Acta 5: 757, 1960), routinely used in our laboratories, by analysis of 82 samples of cerebrospinal fluid (CSF) and 119 samples of urine. Our results for cerebrospinal fluid agree well with those of Iwata and Nishikaze (r = 0.976; y = 0.992x - 0.013), but we find their method unsuitable for urinary protein determination, probably because of interfering compounds in urine.
Authors:
E Flachaire; O Damour; J Bienvenu; T Aouiti; R Later
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Clinical chemistry     Volume:  29     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1983 Feb 
Date Detail:
Created Date:  1983-03-17     Completed Date:  1983-03-17     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  343-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Albumins / analysis
Benzethonium*
Cerebrospinal Fluid Proteins
Evaluation Studies as Topic
Humans
Immunoglobulins / analysis
Nephelometry and Turbidimetry
Proteins / analysis*
Proteinuria
Quaternary Ammonium Compounds*
Salicylic Acids
Spectrophotometry
Chemical
Reg. No./Substance:
0/Albumins; 0/Cerebrospinal Fluid Proteins; 0/Immunoglobulins; 0/Proteins; 0/Quaternary Ammonium Compounds; 0/Salicylic Acids; 121-54-0/Benzethonium; 97-05-2/sulfosalicylic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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