Document Detail

Assessment of an association between an aryl hydrocarbon receptor gene (AHR) polymorphism and risk of male infertility.
MedLine Citation:
PMID:  21613234     Owner:  NLM     Status:  Publisher    
The aryl hydrocarbon receptor (AHR) is a ligand activated transcription factor that mediates the toxicity of a variety of environmental chemicals, such as polycyclic aromatic hydrocarbons (PAHs) and dioxins. We hypothesized that polymorphisms of AHR may result in significant differences in sensitivity to toxic effects of PAHs or dioxins and contribute to susceptibility to male infertility. To address this possibility, we conducted a study including 580 idiopathic infertile subjects and 580 fertile controls to assess associations between the male infertility risk and six tagging SNPs (tagSNPs) of AHR gene. Additionally, correlations between AHR polymorphisms and sperm concentration, levels of DNA fragmentation and BPDE-DNA adducts in sperm were determined in 420 patients. Genotypes were determined using the ABI OpenArray platform. Sperm DNA fragmentation was evaluated by TdT-mediated dUDP nick-end labelling (TUNEL) assay and sperm BPDE-DNA adducts were measured by immunofluorescent assay using flow cytometry. We found that the G variant of rs2158041 was associated with significantly increased risk of male infertility [adjusted odds ratio (OR), 1.53; 95% confidence intervals (CI), 1.21-1.93; P = 6.0×10(-6); GA/AA versus GG genotypes]. Furthermore, patients with rs215804 AA genotype showed a reduced sperm concentration. In addition, a gradual increase of sperm DNA fragmentation and sperm BPDE-DNA adducts was found among the three rs2158041 subgroups (GG -> GA -> AA), though the differences were not statistically significant. These results suggested that the AHR polymorphism might be associated with individual risk of male infertility in the Chinese population study.
Aihua Gu; Guixiang Ji; Yan Long; Yong Zhou; Xiangguo Shi; Ling Song; Xinru Wang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-5-24
Journal Detail:
Title:  Toxicological sciences : an official journal of the Society of Toxicology     Volume:  -     ISSN:  1096-0929     ISO Abbreviation:  -     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-5-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9805461     Medline TA:  Toxicol Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
State Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing, 210029, China.
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